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Highly Morphogenic Callus of Arachis Paraguariensis from Agar and Suspension Cultures

  • Paul E. Still
  • María Inés Plata
  • C. L. Niblett
Chapter
Part of the Basic Life Sciences book series (BLSC, volume 32)

Abstract

Arachis paraguariensis (Chod. et Hassl.) is a wild species of peanut with 2n=20. Callus cultures derived from anthers of this species were highly morphogenic, producing shoots, leaves, and flowers at a high frequency. A much lower percentage regenerated into complete plants with roots. Anthers from flowers with no petal showing were used to initiate callus, using the salts and organics of N6 medium (Chu et al.) supplemented with casein hydrolysate, 500 mg/1; myo-inositol, 100 mg/1; sucrose, 20 g/1; L-proline, 3 g/1; 4-amino-3,5,6-trichloropicolinic acid (picloram), 0.2 mg/1; N6-benzyladenine (BA), 0.25 mg/1; and agar, 6 g/1. Calli were maintained on the same N6 medium with picloram levels of 0.008 mg/1, 0.25 mg/1 BA, and 60 g/1 sucrose. Buds appeared on calli after 30 to 40 days. When these buds were transferred to Murashige and Skoog medium (MS) with 0.01 mg/1 BA or with no hormones, shoots developed with leaves and flowers. Callus was used to initiate suspension cultures in N6 liquid medium that contained casein hydrolysate, 500 mg/1; myo-inositol, 100 mg/1; sucrose, 20 g/1; picloram, 0.008 mg/1; BA, 0.2 mg/1; and L-proline, 1.7 g/1. Suspension cultures were maintained by transferring clumps of tissue between 210 and 840 μm in size on a weekly basis.

Keywords

Tissue Culture Plant Pathology Lower Percentage Suspension Culture Wild Species 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • Paul E. Still
    • 1
  • María Inés Plata
    • 1
  • C. L. Niblett
    • 1
  1. 1.Department of Plant PathologyUniversity of FloridaGainesvilleUSA

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