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Cell Tension and Cavitation in Plants During Freezing

Their Role in Injury
  • C. B. Rajashekar

Abstract

Freezing behavior of water in plant tissues is often thought to be similar to that of dilute aqueous solutions. Typically, plant tissue water freezes extracellularly which requires cell deformation, specifically, cell contraction. Considering that cells are bound by often rigid cell walls, it is likely that they would offer some resistance to volume changes. This clearly can affect the freezing of water in plant tissues. As cells shrink during extracellular freezing, it allows for cell dehydration to occur (Levitt, 1980). However, when cells offer resistance to deformation during extracellular freezing it can lead to reduced cell dehydration. The impact of cell resistance to deformation during freezing is evident in extreme cases such as supercooling cells which do not dehydrate much, despite the presence of ice in rest of the tissue (George and Burke, 1977). This is remarkable considering the fact that just above the homogeneous nucleation temperature, the water potential of ice surrounding the supercooling cells could be as low as −46 MPa. Also, recent evidence suggests that even non-supercooling woody tissues do not shrink as expected during extracellular freezing (Malone and Ashworth, 1991). Our studies and those of others have shown that even herbaceous tissues such as leaves can resist cell deformation and dehydration during extracellular freezing (Rajashekar and Burke, 1982; Anderson et al., 1983; Hansen and Beck, 1988; Zhu and Beck, 1991; Rajashekar and Lafta, 1996).

Keywords

Acoustic Emission Bulk Modulus Cold Acclimation Homogeneous Nucleation Unfrozen Water 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Akopyan VB (1980) Determination of the cavitation threshold in biological tissue from the luminescence appearing on exposure to ultrasound. Biofizika 25: 873–876Google Scholar
  2. Anderson JA, Gusta LV, Buchanan DW, Burke MJ (1983) Freezing of Water in citrus leaves. J Amer Soc Hort Sci 108: 397–400Google Scholar
  3. Barber BP, Wu C, Lofstedt R, Roberts PH, Putterman SJ (1994) Sensitivity of sonoluminescence to experimental parameters. Phy Rev Lett 72: 1380–1383CrossRefGoogle Scholar
  4. Cataldo FL, Miller MW, Gregory WD (1973) A description of ultrasonically-induced chromosomal anomolies in Vicia faba. Radiation Bot 13: 211–213CrossRefGoogle Scholar
  5. Chen THH, Gusta LV (1983) Abscisic acid-induced freezing resistance in cultured plant cells. Plant Physiol 73: 71–75PubMedCrossRefGoogle Scholar
  6. George MF, Burke MJ (1977) Cold hardiness and deep supercooling in xylem of shagbark hickory. Plant Physiol 59: 319–325PubMedCrossRefGoogle Scholar
  7. Green JL, Durben DJ, Wolf GH, Angeli CA (1990) Water and solutions at negative pressure: Raman spectro-scopic study to −80 megapascals. Science 249: 649–652PubMedCrossRefGoogle Scholar
  8. Gusta LV, Burke MJ, Kapoor AC (1975) Determination of unfrozen water in winter cereals at subfreezing temperatures. Plant Physiol 56: 707–709PubMedCrossRefGoogle Scholar
  9. Gusta LV, Tyler NJ, Chen TH (1983) Deep undercooling in woody taxa growing north of −40°C isotherm. Plant Physiol 72: 122–128PubMedCrossRefGoogle Scholar
  10. Hansen J, Beck E (1988) Evidence for ideal and non-ideal equilibrium freezing of leaf water in frost-hardy ivy (Hedera helix) and winter barley (Hordeum vulgare). Botanica Acta 101: 76–82Google Scholar
  11. Henderson SJ, Speedy RJ (1987) Temperature of maximum density in water at negative pressure. J Phys Chem 91:3062–3068CrossRefGoogle Scholar
  12. Hiller R, Putterman SJ, Barber BP (1992) Spectrum of synchronous picosecond sonoluminescence. Phys Rev Lett 69: 1182–1184PubMedCrossRefGoogle Scholar
  13. Huner NPA, Palta JP, Li PH, Carter JV (1981) Anatomical changes in leaves of puma rye in response to growth at cold-hardening temperatures. Bot Gaz 142: 55–62CrossRefGoogle Scholar
  14. Kanno H, Angell CA (1977) Homogeneous nucleation and glass formation in aqueous alkali halide solutions at high pressures. J Phys Chem 81: 2639–2643CrossRefGoogle Scholar
  15. Lehmann JF, Herrick JF, Krusen FH (1954) The effects of ultrasound on chromosomes, nuclei and other structures of the cells in plant tissues. Arch Phys Med Rehab 35: 141–148Google Scholar
  16. Levitt J (1980) Responses of Plants to Environmental Stresses. Academic Press, New YorkGoogle Scholar
  17. Malone SR, Ashworth EN (1991) Freezing stress response in woody tissues observed using low-temperature scanning electron microscopy and freeze substitution techniques. Plant Physiol 95: 871–881PubMedCrossRefGoogle Scholar
  18. Nyborg WL(1978) Physical mechanisms for biological effects of ultrasound. HEW Pub (FDA)78-8062Google Scholar
  19. Pearce RS (1988) Extracellular ice and cell shape in frost-stressed cereal leaves: A low temperature scanning-electro-microscopy study. Planta 175: 313–324CrossRefGoogle Scholar
  20. Rajashekar C, Burke MJ (1978) The occurrence of deep supercooling in the genera Pyrus, Prinus and Rosa, a preliminary report. In PH Li and A Sakai eds, Plant Cold Hardiness and Freezing Stress: Mechanisms and Crop Implications. Vol 1. Academic Press, New York, pp 213–225CrossRefGoogle Scholar
  21. Rajashekar CB, Burke MJ (1982) Liquid water during slow freezing based on cell water relations and limited experimental testing. In PH Li, A Sakai eds, Plant Cold Hardiness and Freezing Stress, Vol 2. Academic Press, New York, pp 211–220CrossRefGoogle Scholar
  22. Rajashekar CB, Burke MJ (1996) Freezing characteristics of rigid plant tissues, Development of cell tension during extracellular freezing. Plant Physiol 111: 597–603PubMedGoogle Scholar
  23. Rajashekar CB, Lafta A (1996) Cell wall changes and cell tension in response to cold acclimation and exogenous abscisic acid in leaves and cell cultures. Plant Physiol 111: 605–612PubMedGoogle Scholar
  24. Raschi A, Mugnozza GS, Surace R, Valentini R, Vazzana C (1989) The use of ultrasound to monitor freezing and thawing of water in plants. Agric Ecosyst Environ 27:411–418CrossRefGoogle Scholar
  25. Rasmussen DH, Mackenzie AP (1972) Effect of solute on ice-solution interfacial free energy; calculation from measured homogeneous nucleation temperatures. In HHG Jellinek, ed, Water Structure at the Water-Polymer Interface. Plenum Press, New York, pp 126–145CrossRefGoogle Scholar
  26. Reaney MJT, Gusta LV (1987) Factors influencing the induction of freezing tolerance by abscisic acid in cell suspension cultures of Bromus inermis Leyss and Medicago sativa L. Plant Physiol 83: 423–427PubMedCrossRefGoogle Scholar
  27. Robertson AJ, Gusta LV, Reaney MJT, Ishikawa M (1987) Protein synthesis in bromegrass (Bromus inermis Leyss) cultured cells during the induction of frost tolerance by abscisic acid or low temperature. Plant Physiol 84:1331–1336PubMedCrossRefGoogle Scholar
  28. Singh J, Miller RW (1985) Biophysical and ultrastructural studies of membrane alterations in plant cells during extracellular freezing: molecular mechanism of membrane injury. In KK Kartha, ed, Cryopreservation of Plant Cells and Organs. CRC Press, Inc., Florida, pp 61–73Google Scholar
  29. Speedy RJ (1982) Stability-limit conjecture. An interpretation of the properties of water. J Phys Chem 86: 982–991Google Scholar
  30. Tyree MT, Dixon MA (1983) Cavitation events in Thuja occidentalis L. Plant Physiol 72: 1094–1099PubMedCrossRefGoogle Scholar
  31. Wallner SJ, Wu M, Anderson-Krengel SJ (1986) Changes in extracellular polysaccharides during cold acclimation of cultured pear cells. J Am Soc Hort Sci 111:769–773Google Scholar
  32. Walton AJ, Reynolds, GT (1984) Sonoluminescence. Adv Phys 33: 595–660CrossRefGoogle Scholar
  33. Weiser RL, Wallner SJ, Waddell JW (1990) Cell wall and extensin mRNA changes during cold acclimation of pea seedlings. Plant Physiol 93:1021–1026PubMedCrossRefGoogle Scholar
  34. Weiser RL, Wallner SJ (1988) Freezing woody plant stems produces acoustic emissions. J Am Soc Hort Sci 113:636–639Google Scholar
  35. Young FR (1989) Cavitation. McGraw-Hill Book company, LondonGoogle Scholar
  36. Zheng Q, Durben DJ, Wolf GH, Angell CA (1991) Liquids at large negative pressures: Water at homogeneous nucleation limit. Science 254: 829–832PubMedCrossRefGoogle Scholar
  37. Zhu JJ, Beck E (1991) Water relations of Pachysandra leaves during freezing and thawing. Evidence of negative pressure potential alleviating freeze-dehydration stress. Plant Physiol 97: 1146–1153PubMedCrossRefGoogle Scholar
  38. Zhu JJ, Steudle E, Beck E (1989) Negative pressures produced in an artificial osmotic cell by extracellular freezing. Plant Physiol 91: 1454–1459PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • C. B. Rajashekar
    • 1
  1. 1.Division of HorticultureKansas State UniversityManhattanUSA

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