Abstract
Published tissue immunoblot protocols for detecting Neotyphodium coenophialum are for polyclonal antibodies (Gwinn et al., 1991; An et al. 1993) and require approximately 24 h to conduct. Commercial acceptance of immunoblot technology may be more widespread if the protocol could be shortened to accommodate labor costs and/or scheduling. The objective of this experiment was to evaluate the time requirements of the tissue immunoblot procedure and determine the minimum amount of time required to give accurate and consistent results.
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An, Z.-Q., M.R. Seigel, W. Hollin, H.-F. Tsai, D. Schmidt, and C.L. Schardl. 1993. Relationships among non-Acremonium sp. fungal endophytes in five grass species. Appl. Environ. Microbiol. 59: 1540–1548.
Gwinn, K.D., M.H. Collins-Shepard, and B.B. Reddick. 1991. Tissue print-immunoblot, an accurate method for the detection of Acremonium coenophialum in tall fescue. Phytopathology. 81: 747–748.
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© 1997 Springer Science+Business Media New York
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Hiatt, E.E., Hill, N.S., Bouton, J.H. (1997). Tissue Immunoblot Procedure for Detection of Neotyphodium Coenophialum in Tall Fescue. In: Bacon, C.W., Hill, N.S. (eds) Neotyphodium/Grass Interactions. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0271-9_48
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DOI: https://doi.org/10.1007/978-1-4899-0271-9_48
Publisher Name: Springer, Boston, MA
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Online ISBN: 978-1-4899-0271-9
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