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High-Yield Recombinant Cytochrome P450 Scc and Its Optimization for Bioelectronics from AB Initio Considerations

  • P. Paschkevitch
  • V. Sivozhelezov
  • S. Vakula
  • V. Sidorovich
  • M. Eldarov
  • F. Nozza
  • C. Nicolini
Part of the Electronics and Biotechnology Advanced (EL.B.A.) Forum Series book series (ELBA, volume 2)

Abstract

Metalloproteins play diverse and important roles in various vital bioenergetic reactions and metabolic pathways, including respiration, photosynthesis, monooxygenation reactions and many others [Harison P.M., 1985]. They have been chosen as the most plausible materials for application in bioelectronics on the basis of several of their well-documented properties discussed in [Nicolini, 1996, Sivozhelezov et al., in preparation] of which the most important are, on the one hand, the ability to use electron as information carrier in the designed molecular electronic devices, and, on the other hand, the pronounced anisotropy ofphysical properties allowing to consider these proteins as cellular automata [Sivozhelezov et al., in preparation] characterized by finite numbers of functional states separated by energy barriers high enough to be overcome by thermal fluctuations. Those anisotropic properties include the presence of highly selective pathways for electron transfer and highly asymmetric distributions of electrostatic fields and hydrophilic/hydrophobic amino acid residues over their surfaces, and can also be used for controlled association of those proteins into supramolecular structures like thin films suitable for macroscopic characterization and/or direct industrial applications [Nicolini, 1996].

Keywords

Killer Toxin Mitochondrial Import Molecular Electronic Device Export Machinery Adrenodoxin Reductase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • P. Paschkevitch
    • 2
  • V. Sivozhelezov
    • 2
  • S. Vakula
    • 2
  • V. Sidorovich
    • 1
  • M. Eldarov
    • 1
  • F. Nozza
    • 3
  • C. Nicolini
    • 3
  1. 1.Center “Bioengineering”Russian Academy of Sciences ProspMoscowRussia
  2. 2.EL.B.A. FoundationMarcianaItaly
  3. 3.Institute of BiophysicsUniversity of GenoaGenoaItaly

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