Transcellular Biosynthesis of Arachidonic Acid Metabolites: From in Vitro Investigations to in Vivo Reality
In the seventies as well as in the early eighties, studies were designed to establish the oxidative pathways of the arachidonic acid by specific cell types in order to determine the structure of end-products generated by a given cell. This approach has delineated specific enzymic patterns by certain cells in the blood and in the vasculature (Table I). Platelets produce mainly 12-hydroxy-eicosatetraenoic acid, 12-hydroxy-heptadecatrienoic acid and thromboxane A2. Endothelial cells synthesize mainly prostaglandin I2 (prostacyclin) and prostaglandins E2 and F2 depending on the site of the vasculature. Polymorphonuclear granulocytes synthesize nearly exclusively leukotriene B4 and eosinophils generate leukotriene C4. Monocytes/macrophages, depending upon their maturation produce both leukotrienes and thromboxane or prostacyclin. In contrast, red blood cells and lymphocytes, devoid of oxygenases cannot generate eicosanoids.
KeywordsArachidonic Acid Metabolite Calcium Ionophore A23187 Prostaglandin Endoperoxide Unstable Intermediate Acceptor Cell
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