Metabolism of Eicosanoids in Mammalian Cells
Eicosanoid lipid mediators (prostaglandins, thromboxanes, and leukotrienes) play an important role in mammalian biochemistry serving as chemical substances of intracellular communication and cellular activation. Discoveries of their biological activities and roles in normal physiology and pathophysiology are legion. An important question to consider with such biologically active compounds is how activity is terminated. For eicosanoids, metabolism is the primary mechanism by which these substances are removed from tissues and ultimately the body. Therefore, a central facet in studying the biochemistry of lipid mediators has been investigations into the means by which eicosanoids are metabolically converted into other compounds and subsequently eliminated.
KeywordsUrinary Metabolite Entry Rate COOH COOH Human Polymorphonuclear Leukocyte Unique Metabolite
Unable to display preview. Download preview PDF.
- 1.Granstrom, E. Two-dimensional thin-layer chromatography of prostaglandins and related compounds. Methods of Enzymology, 86:485–504, 1982.Google Scholar
- 6.Hansen, H.S. Purification and characterization of a 15-keto prostaglandin delta 13-reductase from bovine lung. Biochim. Biophys. Acta 547:136–145 (1979).Google Scholar
- 7.Watanabe, T., Shimizu, T., Iguchi, S., Wakatsuka, H., Hayashi, M., and Hayaishi, O. An NADP-linked prostaglandin D dehydrogenase in swine brain. J. Biol. Chem. 250:548–552 (1975).Google Scholar
- 12.Aoyama, T., Hardwick, J.P., Imaoka, S., Funae, Y., Gelboin, H.V., and Gonzalez, F.J. Clofibrate-inducible rat hepatic P450s IVA1 and IVA3 catalyze the ω-and (ω-l)-hydroxylation of fatty acids and the ω-hydroxylation of prostaglandins E1 and F2a. J. Lipid Res. 31:1477–1482 (1990).PubMedGoogle Scholar
- 20.Nagaoka, I., Yamada, M., Kira, S., and Yamashita, T. Comparative studies on the leukotriene D4-metabolizing enzyme of different types of leukocytes. Comp. Biochem. Physiol. 89B:375–380 (1988).Google Scholar
- 22.Ishikawa, T., Muller, M., Klunemann, C., Schaub, T., and Keppler, D. ATP-dependent primary active transport of cysteinyl leukotrienes across liver canalicular membrane. Role of the ATP-dependent transport system for glutathione S-conjugates. J. Biol. Chem. 265:19279–19286 (1990).PubMedGoogle Scholar
- 34.Sumimoto, H. and Minakami, S. Oxidation of 20-hydroxyleukotriene B4 to 20-carboxyleukotriene B4 by human neutrophil microsomes. Role of aldehyde dehydrogenase and leukotriene B4 ω-hydroxylase (cytochrome P-450LTBω) in leukotriene B4 ω-oxidation. J. Biol. Chem. 265:4348–4353 (1990).PubMedGoogle Scholar
- 40.Yokomizo, T., Izumi, T., Takahashi, T., Kasama, T., Kobayashi, Y., Sato, F., Taketani, Y., and Shimizu, T. Enzymatic inactivation of leukotriene B4 by a novel enzyme found in the porcine kidney. Purification and properties of leukotriene B4 12-hydroxydehydrogenase. J. Biol. Chem. 268:18128–18135 (1993).PubMedGoogle Scholar
- 43.Wheelan, P. and Murphy, R.C.: Metabolism of 6-trans isomers of leukotriene B4 in cultured hepatoma cells and in human polymorphonuclear leukocytes: Identification of a Δ6-reductase metabolic pathway. J. Biol. Chem., in press.Google Scholar