Advertisement

Taurine 2 pp 393-400 | Cite as

Characterization of the Volume-Activated Taurine Pathway in Cultured Cerebellar Granule Neurons

  • H. Pasantes-Morales
  • C. Peña Segura
  • O. García
  • M. M. Morales Mulia
  • R. Sánchez Olea
  • J. Morán
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 403)

Abstract

Early studies on the mechanism of cell taurine transport have consistently identified a component of taurine uptake which is nonsaturable, energy-independent and clearly corresponding to a diffusional mechanism. For long time this component was normally discarded in all studies of taurine uptake in order to obtain the saturable curves of the energy-dependent, high affinity component, which was associated with a presumed neurotransmitter function for taurine. The recent studies on a role for taurine as an osmolyte have praised the diffusional component of the transport system as it has been identified as the mechanism allowing a rapid extrusion of the amino acid to regulate, together with other osmolytes, the cell water content. The occurrence and features of the two components of the taurine transport system fit very adequately this osmolyte role: a diffusional, rapid release of intracellular pools corrects almost immediately an excess of cell water and after this regulatory function, the intracellular taurine pools are replenished by the energy-dependent, high affinity component, which is able to accumulate taurine against large concentration gradients.

Keywords

Anion Channel Regulatory Volume Decrease Niflumic Acid Cell Volume Regulation Taurine Transport 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Banderali, U. and Roy, G. 1992, Anion channels for amino acids in MDCK cells, Am.J.Physiol. 263:C1200–C1207.Google Scholar
  2. 2.
    Benz, R. 1994, Permeation of hydrophilic solutes through mitochondrial outer membranes: review on mitochondrial porins, Biochim.Biophys.Acta, 1197:167–196.CrossRefGoogle Scholar
  3. 3.
    Dermietzel, R., Hwang, T., Buettner, R., Hofer, A., Dotzler, E., Kremer, M., Deutzmann, R., Thinnes, F.P., Fishman, G.I., Spray, D.C., and Siemen, D. 1994, Cloning and in situ localization of a brain-derived porin that constitutes a large conductance anion channel in astrocytic plasma membranes, Proc. NatlAcad. Scl USA, 91:499–503.CrossRefGoogle Scholar
  4. 4.
    Falke, J.J. and Chan, S.I. 1986, Molecular mechanisms of band 3 inhibitors: I transport site inhibitors, Biochemistry, 25:7888–7894.CrossRefGoogle Scholar
  5. 5.
    Goldstein, L. and Brill, S.R. 1991, Volume-activated taurine efflux from skate erythrocytes: Possible band 3 involvement, Am.J.Physiol. 260:R1014–R1020.Google Scholar
  6. 6.
    Hallows, K.R. and Knauff, P.A. 1994, Principles of cell volume regulations, in: “Cellular and Molecular Physiology of Cell Volume Regulation”, Strange, K. ed., CRC Press, Boca Raton, pp. 3–30.Google Scholar
  7. 7.
    Hoffmann, E.K. and Simonsen, L.O. 1989, Membrane mechanisms in volume and pH regulation in vertebrate cells, Physiol.Rev. 69:315–382.Google Scholar
  8. 8.
    Hollt, V, Kouba, M., Dietel, M., and Vogt, G. 1992, Steroisomers of calcium antagonists which differ markedly in their potencies as calcium blockers are equally effective in modulating drug transport by p-glycoprotein, Biochem.Pharmacol. 3:2601–2608.CrossRefGoogle Scholar
  9. 9.
    Jackson, P.S. and Strange, K. 1993, Volume-sensitive anion channels mediate swelling-activated inositol and taurine efflux, Am.J.Physiol. 265:C1489–C1500.Google Scholar
  10. 10.
    Jentsch, T. 1994, Molecular physiology of anion channels, Curr.Opin.Cell Biol. 6:600–606.CrossRefGoogle Scholar
  11. 11.
    Kay, M.M.B., Hughes, J., Zagón, I., and Lin, F. 1991, Brain membrane protein 3 performs the same functions as erythrocyte band 3, Proc.Natl.Acad.Sci.USA, 88:2778–2782.CrossRefGoogle Scholar
  12. 12.
    Lehmann, A. 1990, Derangements in cerebral osmohomeostasis: A common denominator for stimulation of taurine and phosphoethanolamine release, in: “Taurine: Functional Neurochemistry, Physiology, and Cardiology”, Pasantes-Morales, H., Martin, D.L., Shain, W. and del Río, R.M. eds., Wiley-Liss, New York, pp. 337–347.Google Scholar
  13. 13.
    Ordway, R.W., Singer, J.J., and Walsh, J.V. 1991, Direct regulation of ion channels by fatty acids, Trends Neurosci. 14:96–100.CrossRefGoogle Scholar
  14. 14.
    Pasantes-Morales, H., Chacón, E., Murray, R.A., and Morán, J. 1994, Properties of osmolyte fluxes activated during regulatory volume decrease in cultured cerebellar granule neurons, J.Neurosci.Res. 37:720–727.CrossRefGoogle Scholar
  15. 15.
    Pasantes-Morales, H. and Del Río, R.M. 1990, Taurine and mechanisms of cell volume regulation, in: “Taurine: Functional Neurochemistry, Physiology, and Cardiology”, Pasantes-Morales, H., Martin, D.L., Shain, W. and del Río, R.M. eds., Wiley-Liss, New York, pp. 317–328.Google Scholar
  16. 16.
    Paulmich, M., Gschwentner, M., Woll, E., Schmarda, A., Ritter, M., Kanin, G., Ellemunter, H., Waitz, W., and Deetjen, P. 1993, Insight into the structure-function relation of chloride channels, Cell Physiol.Biochem. 3:374–387.CrossRefGoogle Scholar
  17. 17.
    Sanchez-Olea, R., Morán, J., Schousboe, A., and Pasantes-Morales, H. 1991, Hyposmolarity-activated fluxes of taurine in astrocytes are mediated by diffusion, Neurosci.Lett. 130:233–236.CrossRefGoogle Scholar
  18. 18.
    Sánchez-Olea, R., Morales-Mulia, M., Morán, J., and Pasantes-Morales, H. 1995, Inhibition by polyunsaturated fatty acids of cell volume regulation and osmolyte fluxes in astrocytes, Am.J.Physiol. 269:C96–C102.Google Scholar
  19. 19.
    Sánchez-Olea, R., Morán, J., and Pasantes-Morales, H. 1992, Changes in taurine transport evoked by hyperosmolarity in cultured astrocytes, J.Neurosci.Res. 32:86–92.CrossRefGoogle Scholar
  20. 20.
    Sánchez-Olea, R., Peña, C., Morán, J., and Pasantes-Morales, H. 1993, Inhibition of volume regulation and efflux of osmoregulatory amino acids by blockers of Cl- transport in cultured astocytes, Neurosci.Lett. 156:141–144.CrossRefGoogle Scholar
  21. 21.
    Schousboe, A., Sánchez Olea, R., Morán, J., and Pasantes-Morales, H. 1991, Hyposmolarity-induced taurine release in cerebellar granule cells is associated with diffusion and not with high-affinity transport, J.Neurosci.Res. 30:661–665.CrossRefGoogle Scholar
  22. 22.
    Solís, J.M., Herranz, A.S., Herreras, O., Lerma, J., and Del Río, R.M. 1988, Does taurine act as an osmoregulatory substance in the rat brain, Neurosci.Lett. 91:53–58.CrossRefGoogle Scholar
  23. 23.
    Thurston, J.H., Hauhart, R.E., and Dirgo, J.A. 1980, Taurine: a role in osmotic regulation of mammalian brain and possible clinical significance, Life Sci. 26:1561–1568.CrossRefGoogle Scholar
  24. 24.
    Trachtman, H., Futterweit, S., and Del Pizzo, R. 1992, Taurine and osmoregulation. IV. Cerebral taurine transport is increased in rats with hypernatremic dehydration, Pediatr.Res. 32:118–124.CrossRefGoogle Scholar
  25. 25.
    Tratchman, H., Barbour, R., and Sturman, J.A. 1988, Taurine and osmoregulation: taurine is a cerebral osmoprotective molecule in chronic hypernatremic dehydration, Pediatr.Res. 23:35–41.CrossRefGoogle Scholar
  26. 26.
    Valverde, M.A., Diaz, M., Gill, F.V, Hyde, S.C., and Higgins, C.F. 1992, Volume-regulated chloride channels associated with the human multidrug resistance p-glycoprotein, Nature, 355:830–833.CrossRefGoogle Scholar
  27. 27.
    Wade, J.V, Olson, J.P., Samson, F.E., Nelson, S.R., and Pazdernik, T.L. 1988, A possible role for taurine in osmoregulation within the brain, J.Neurochem. 51:740–745.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • H. Pasantes-Morales
    • 1
  • C. Peña Segura
    • 1
  • O. García
    • 1
  • M. M. Morales Mulia
    • 1
  • R. Sánchez Olea
    • 1
  • J. Morán
    • 1
  1. 1.Institute of Cell PhysiologyNational University of MexicoMexico CityMexico

Personalised recommendations