Advertisement

Taurine 2 pp 343-350 | Cite as

Effects of Kinase Inhibitors and Taurine Analogues on the Phosphorylation of Specific Proteins in Mitochondrial Fractions of Rat Heart and Retina

  • John B. Lombardini
  • Cristi Props
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 403)

Abstract

We have demonstrated that taurine at physiological concentrations inhibits the phosphorylation of specific proteins in mitochondrial fractions of the rat heart and retina2, 5. However, while the functions of these proteins, specifically an ∼44 kDa phosphoprotein present in the heart and an ∼20 kDa phosphoprotein present in the retina, are unknown we have considered it worthwhile to characterize these proteins with respect to a number of parameters. The concentration-response relationships of the effects of taurine and a series of taurine analogues on the phosphorylation of both the ∼44 kDa5 and ∼20 kDa proteins have been studied. In addition, we have characterized both proteins with respect to the nature of the phosphate bond, the identification of the phosphorylated amino acid, the isoelectric point of the proteins, and the partition of the proteins into an aqueous phase rather than a lipid phase3, 4, 5.

Keywords

Kinase Inhibitor Mitochondrial Fraction Combination Index Calmodulin Antagonist Phosphorylated Amino Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Chou, T.-C. and Talalay, P., 1986, Applications of the median-effect principle for the assessment of low-dose risk of carcinogens and for the quantitation of synergism and antagonism of chemotherapeutic agents, in: “New Advances in Developmental Cancer Therapy”, Bristol-Meyers Cancer Symposia, Harrap, K.R. and Conners, T.A., eds., Academic Press, New York, pp. 37–64.Google Scholar
  2. 2.
    Lombardini, J.B., 1992, Effects of taurine on the phosphorylation of specific proteins in subcellular fractions of the rat retina, Neurochem. Res., 17:821–824.CrossRefGoogle Scholar
  3. 3.
    Lombardini, J.B., 1993, Partial characterization of an ∼20 K Mr retinal protein whose phosphorylation is inhibited by taurine, Biochem. Pharmacol, 46:1445–1451.CrossRefGoogle Scholar
  4. 4.
    Lombardini, J.B., 1994, The inhibitory effects of taurine on protein phosphorylation: Comparisons of various characteristics of the taurine-affected phosphoproteins present in rat retina, brain and heart, in Adv. Exp. Med. Biol. Taurine in Health and Disease, Huxtable, R.J. and Michalk, D.V., eds., Plenum Press, New York, Vol. 359, pp 9–17.Google Scholar
  5. 5.
    Lombardini, J.B., 1994, Inhibitory effects of taurine and taurine analogues on the phosphorylation of an ∼44 K Mr protein present in a mitochondrial subfraction of the rat heart: partial characterization of the ∼44 K Mr phosphoprotein, J. Mol Cell Card., 26:1675–1689.CrossRefGoogle Scholar
  6. 6.
    Lombardini, J.B., 1995, Paradoxical stimulatory effect of the kinase inhibitor chelerythrine on the phosphorylation of an ∼20 K Mr protein present in the mitoichondrial fraction of rat retina, Brain Res., 46:1445–1451.Google Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • John B. Lombardini
    • 1
    • 2
  • Cristi Props
    • 1
  1. 1.Department of PharmacologyTexas Tech University Health Sciences CenterLubbockUSA
  2. 2.Department of Ophthalmology and Visual SciencesTexas Tech University Health Sciences CenterLubbockUSA

Personalised recommendations