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PAF-Induced MAPK Activation is Inhibited by Wortmannin in Neutrophils and Macrophages

  • Ingvar Ferby
  • Iwao Waga
  • Kazuhiko Kume
  • Chie Sakanaka
  • Takao Shimizu
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 416)

Abstract

The signal transduction mediating PAF-induced MAPK activation in CHO-cells stably expressing cloned PAF-receptor, has been extensively studied in our group (1). Pertussis toxin (PTX) blocked about 50% of the PAF-induced MAPK activation, without altering phosphatidylinositol (PI) hydrolysis and calcium responses. It was therefore speculated that the PAF-receptor mediates MAPK activation through one PTX-insensitive pathway that yields PI hydrolysis and possibly activation of conventional protein kinase C (cPKC), and another PTX-sensitive pathway that is likely to be independent of calcium and cPKC but mainly remains obscure.

Keywords

Phorbol Myristate Acetate MAPK Activation Calcium Response Phorbol Myristate Acetate Intracellular Calcium Chelator 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Ingvar Ferby
    • 1
  • Iwao Waga
    • 1
  • Kazuhiko Kume
    • 1
  • Chie Sakanaka
    • 1
  • Takao Shimizu
    • 1
  1. 1.Department of Biochemistry, Faculty of MedicineThe University of TokyoBunkyo-ku, Tokyo 113Japan

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