PAF Promotes the Development of the Preimplantation Embryo
The preimplantation embryo of mammalian species represents the period from fertilisation until the implantation of the embryo into the uterus at the blastocyst stage. In species such as experimental rodents and humans, implantation occurs 4–6 days after fertilisation at which time the embryo has 60–100 cells. During this time the embryo remains free-living within the reproductive tract and there is an exponential increase in cell numbers. Experiments in vitro have shown that growth and development of the embryo during this period is relatively autonomous requiring simple defined medium. There is no apparent absolute requirement for exogenous growth factors. Such autonomy suggests that the stimulus for growth during this period may be endogenous to the embryo.
KeywordsMitotic Index Platelet Activate Factor Reproductive Tract Preimplantation Embryo Preimplantation Mouse Embryo
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- 3.Ammit AJ, O’Neill C, 1995. PAF released by preimplantation embryos binds to albumin. In: Nigam S, 3. Kunkel G, Prescott SM, Vargaftig BB (eds.), Platelet-activating factor and related lipid mediators in health and disease. New York: Plenum PublishingGoogle Scholar
- 5.Wells XE, O’Neill C, 1994. Detection and preliminary characterization of two enzymes involved in biosynthesis of platelet-activating factor in mouse oocytes,zygotes and preimplantation embryos: dithiothreitol-insensitive cytidinediphospho-choline: i-o-alkyl-2-acetyl-sn-glycerol cholinephosphotransferase and acetyl-coenzyme A: 1-o-alkyl-2-lyso-sn-glycero-3-phosphocholine acetyltransferase. J Reprod Fertil 101: 385–391CrossRefGoogle Scholar
- 9.O’Neill C, Ryan JP, Collier M, Saunders DM, Ammit AJ, Pike IL, 1989. Supplementation of IVF culture media with platelet activating factor (PAF) increased the pregnancy rate following embryo transfer. Lancet ii: 769–772Google Scholar