Platelet-Activating Factor and Angiogenesis
Platelet-activating factor (PAF) is a phospholipid autacoid with a spectrum of diverse and potent biological properties relevant for the development of inflammatory reaction and septic shock1. Recently, PAF has been also implicated in the embryogenesis and cell differentiation2,3. Researchers believe that PAF is a mediator of cell to cell communication which may function both as intercellular and intracellular messenger. Since PAF is produced after appropriate stimulation by many cell types, including most inflammatory cells, it has been implicated in the pathogenesis of a variety of diseases4. Moreover, human endothelial cells (EC) were found to produce PAF after stimulation by several inflammatory mediators5,6. Many cell types that synthesized PAF, including EC, are also target for PAF7,8 and express PAF-binding sites on their surface9. PAF, indeed, directly stimulates the endothelium, as it enhances the permeability of EC monolayer and induces changes of the cell cytoskeleton, leading to cell retraction and formation of intercellular gaps8. Moreover, it was observed that, in sub-confluent EC cultures, the redistribution of cytoskeleton was characteristic of cells undergoing translational movements8. These observations prompted us to evaluate whether PAF may stimulate the migration of EC in vitro.
KeywordsHepatocyte Growth Factor Platelet Activate Factor Human Endothelial Cell Endothelial Cell Migration Angiogenic Effect
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