Influence of Hexadecylphosphocholine (Miltefosine) on Cytokine Synthesis and Biological Responses
Hexadecylphosphocholine, INN: Miltefosine (Figure 1), the lead compound of the alkylphosphocholines, has shown its antineoplastic activity in various in-vitro and in-vivo tumor models (Hilgard et al. 1993). After oral administration to patients miltefosine induced a remarkable leucocytosis (Pronk et al. 1994). This suggested a stimulatory effect of the compound on the hematopoietic system. To investigate this phenomenon, mouse and rat bone marrow was stimulated with either recombinant mouse/rat IL-3 or mouse GM-CSF with or without the addition of miltefosine. Additionally, Northern blot analysis was performed on human myeloid leukemic cell lines for the IL-3 and GM-CSF receptor genes and for TNFα mRNA on peripheral blood cells stimulated with concanavalin A (Con A) or lipopolysaccharid (LPS) when they were exposed to Miltefosine.
KeywordsNorthern Blot Analysis Cytokine Synthesis Colony Form Unit Assay eDNA Probe Human Myeloid Leukemic Cell Line
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- Baier JE, Brauckmann-Berger JM, Neumann HA (1995) Modulatorische Eigenschaft des Alkyllysophospholipids Ilmofosin auf Zytokine. Tumordiagn. u. Ther. 16, 88–93Google Scholar
- Berger MR, Yanapirut P, Reinhardt M, Klenner T, Scherf HR, Schmeiser HH, Eibl H (1992) Antitumor activity of Alkylphosphocholines and analogues in methylnitrosourea-induced rat mammary carcinomas. In: Eibl H, Hilgard P, Unger C (eds): Alkylphosphocholines: New drugs in Cancer Therapy. Prog Exp Tumor Res. Basel, Karger, Vol. 34, 98–115Google Scholar
- Harlemann J (1994) Tumor differentiation in-vivo with Miltefosine. In: Drugs of Today, Clinical aspects of milte- fosine and ist topical formulation Miltex® Ed: Hilgard P, Engel J, Vol. 30, Suppl. B, 43–46Google Scholar
- Hilgard P, Klenner T, Stekar J, Unger C (1993) Alkylphosphocholines: A new class of membrane-active anticancer agents. Cancer Chemother Pharmacol 32, 90–95Google Scholar
- Nooter K, Stoter G, Verweij J (1994) Induction of hematopoietic differentiation by the alkylphosphocholine Miltefosine. In: Drugs of Today, Clinical aspects of miltefosine and ist topical formulation Miltex® Ed: Hilgard P, Engel J. Vol. 30, Suppl. B, 31–42Google Scholar