Inhibitors of Arachidonate Metabolism and Effects on PAF Production

  • James D. Winkler
  • Chiu-Mei Sung
  • Lisa A. Marshall
  • Floyd H. Chilton
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 416)


Our understanding of the enzyme Coenzyme A-independent transacylase (CoA-IT) has increased dramatically over the last 10 years. The enzyme catalyses the removal of the fatty acyl group from the sn-2 position of glycerophospholipids (GPL) and transfers it into 1-radyl-2-lyso GPL.1 The enzyme shows striking selectivity for transfer of arachidonate and other long-chain, unsaturated fatty acyl groups. It also shows strong preference for phosphocholine-and phosphoethanolamine-containing GPL, along with a preference for using 1-ether GPL as acceptors for the transferred arachidonate.2 The mechanism of action of CoA-IT has yet to be defined at the molecular level, but CoA-IT is hypothesized to be a member of the family of tranferases typified by lecithin-cholesterol acyl transferase.3 Based on these characteristics, CoA-IT has been presumed to play a role in the movement of arachidonate between GPL that occurs in inflammatory cells.4–7


Human Neutrophil Arachidonate Metabolism Trifluoromethyl Ketone Bowman Gray School Methyl Phenoxy 


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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • James D. Winkler
    • 1
  • Chiu-Mei Sung
    • 1
  • Lisa A. Marshall
    • 1
  • Floyd H. Chilton
    • 2
  1. 1.Division of PharmacologySmithKline Beecham PharmaceuticalsKing of PrussiaUSA
  2. 2.Section on Pulmonary and Critical Care Medicine and Department of BiochemistryBowman Gray School of MedicineWinston-SalemUSA

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