Abstract
Platelet-activating factor (PAF) has been implicated in a number of reproductive processes ranging from ovulation to parturition. To examine the role of PAF in the human periovulatory processes, the PAF-acetylhydrolase (PAF-AH) activity was assayed in the follicular fluid (FF) obtained in conjunction with the in vitro fertilization and embryo transfer (IVF-ET) procedure and the activity related to oocyte maturation. The PAF-AH activity was also related to the concentrations of estradiol (E2) and progesterone (P) in FF. PAF-AH activity was significantly lower in the FFs obtained from follicles of more than 20 mm in diameter. The enzyme activity was significantly lower in the FFs of patients with a successful outcome of their pregnancies. E2 concentrations were negatively correlated with PAF-AH activities in the FFs. No correlation was found between the PAF-AH activity and concentration of P in the FF. Significantly more mature oocytes were recovered in the group who subsequently become pregnant compared to the non-pregnant group. It is suggested that PAF may be increased following follicular maturation. The increase in PAF may contribute to oocyte maturation and to the successful outcome of pregnancy following fertilization. An additional function of the increased PAF in FF may also be the stimulation of the contraction of smooth muscle in the ovary, thereby assisting the extrusion of the oocyte cumulus cell mass and signaling the completion of ovulation.
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References
L. Speroff, R.H. Glass, and N.G. Kase, Regulation of the menstrual cycle, in: Clinical Gynecologic Endocrinology and Infertility, L. Speroff, R.H. Glass, N.G. Kase, eds., 4th edition (1989) pp91.
L.L. Espey, Ovulation as an inflammatory reaction: a hypothesis, Biol. Reprod. 22: 73 (1980).
F Snyder, Platelet-activating factor and related acetylated lipids as potent biologically active cellular mechanisms, Am. J. Physio1. 259: C697 (1990).
P. Braquet, L, T.uqui, T.Y. Shen, and B.B. Vargaftig, Perspectives in platelet-activating factor research, Parmacol. Rev. 39: 97 (1987)
S.M. Prescott, G.A. Zimmerman, and T.M. McIntyre, Platelet-activating factor, J. Biol. Chem. 265:17381 (1990);
M.R. Elstad, D.M. Stafforini, K.E. Stremler, G.A. Zimmermen, T.M. McIntyre, and S.M. Prescott, The potential role of the PAF acetylhydrolase in human disease, in. Platelet-Activating Factor and Disease, K. Saito, D.J. Hanahan, eds. International Medical Publishers, Tokyo, Japan: 69 (1989).
A.O. Abisogun, P. Braquet, and A, Tsafriri, The involvement of platelet-activating factor in ovulation, Science 243: 381 (1989).
L.L. Espey, N. Tanaka, D.S. Woodard, M.J.K. Harper, and H. Okamura. Decrease in ovarian platelet-activating factor during ovulation in the gonadotropin-primed immature rat, Biol. Reprod. 41: 104 (1989).
B.M. Alexander, E.A. Van Kirk, and W.J. Murdoch, Secretion of platelet-activating factor by periovulatory ovine follicles, Life Sci 47: 865 (1990).
M.J. Amiel, J. Testart, and J. Benveniste, Platelet-activating factor-acether is a component of human follicular fluid Fertil Steril 56: 62 (1991).
X.-M. Li, N. Sagawa, Y. Ihara, A. Okagaki, H. Hasegawa, K. Inamori, H. Itoh, T. Mori, C. Ban, The involvement of platelet-activating factor in thrombocytopenia and follicular rupture during gonadotropin-induced superovulation in immature rats, Endocrinology 128: 3132 (1991).
B.S. Minhas, Platelet-activating factor treatment of human spermatozoa enhances fertilization potential, Am. J. Obstet. Gynecol. 168: 1314 (1993).
C. O’Neill, PAF and the establishment of pregnancy, in: Platelet-activating factor and human disease, P.C. Barnes, C.P. Page, P.M. Henson, eds… Blackwell, Oxford. 282–296 (1990).
H. Narahara, R.A. Frenkel, and J.M. Johnston, The role of PAF in reproductive biology, in Advances in Molecular Biology-Lipobiology, R. Gross, ed.. JAI Press Inc., Greenwich, CT, in press..
R.S. Fan, M.L. Wardlow, C.P. Cox, K.E. Meng, and D.E. Greene, Human serum acid-labile factor in an acyl-hydrolase that inactivates platelet-activating factor, Fed. Proc. 42: 3120 (1983).
H. Narahara, Y. Nishioka, and J.M. Johnston, Secretion of platelet-activating factor acetylhydrolase by human decidual macrophages, J. Endocrinol. Metab. 77: 1258–62 (1993).
H. Narahara, R. Frenkel, and J.M. Johnston, Secretion of platelet-activating factor acetylhydrolase (PAF-AH) following phorbol ester-stimulated differentiation of HL-60 cells, Arch. Biochem. Biophys. 301: 275 (1993).
S. Miyaura, N. Maki, W. Byrd, and J.M. Johnston, The hormonal regulation of platelet-activating factor acetylhydrolase activity in plasma, Lipids 26: 1015 (1991).
K. Yasuda, and J.M. Johnston, The hormonal regulation of platelet-activating factor acetylhydrolase in the rat, Endocrinology 130: 708 (1992).
K. Yasuda, H. Eguchi, H.Narahara, and J.M. Johnston, Platelet-activating factor: Its regulation in parturition. in: Eicosanoids and Other Bioactive Lipids in Cancer, Inflammation and Radiation Injury, S. Nigam, K.V. Honn, L.J. Marnett, T.L. Walden, eds., Kluwer Academic Publishers, Boston, 727 (1993).
N. Lepage, R Miron, R. Hammings, K.D. Roberts, and J. Langlais, Distribution of lysophaspholipids and metabolism of platelet-activating factor in human follicular and peritoneal fluids J. Reprod. Fertil. 98: 349 (1993).
M. Miwa, T. Miyake, T. Yamanaka, J. Sugatani, Y. Suzuki, S. Sakata, Y. Araki, and M. Matsumoto, Characterization of serum platelet-activating factor (PAF) acetylhydrolase. Correlation between deficiency of serum PAF acetylhydrolase and respiratory symptoms in asthmatic children J. Clin. Invest. 82: 1983 (1988).
J.A. Loukides, R.A. Loy, R. Edwards, J. Hong, I. Visintin, and M.L. Polan, Human follicular fluid contain tissue macrophages, J. Clin. Endocrinol. Metab. 71: 1363 (1990).
R. Nakayama, K. Yasuda, T. Okumura, and K. Saito, Effect of 17ß-estradiol on PAF and prostaglandin levels in oophorectomized rat uterus Biochem. Biophys. Acta 1085: 235 (1991).
J. Rabinovici, and M.J. Angle, Platelet-activating factor induces progesterone secretion and changes in morphological appearance on luteinizing granulosa cells in vitro,. Fertil. Steril. 55: 1106 (1991).
Y. Takehara, A.M. Dharmarajan, G. Kaufman, E.E. Wallach, Effect of interleukin-1 ß on ovulation in the in-vitro perfused rabbit ovary, Endocrinology 134: 1788 (1994).
H. Narahara, and J.M. Johnston, Effects of endotoxins and cytokines on the secretion of platelet-activating factor-acetylhydrolase by human decidual macrophages, Am. J. Obstet. Gynecol. 169; 531 (1993).
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Narahara, H., Tanaka, Y., Kawano, Y., Miyakawa, I., Johnston, J.M. (1996). Platelet-Activating Factor Acetylhydrolase Activity in Human Follicular Fluid. In: Nigam, S., Kunkel, G., Prescott, S.M. (eds) Platelet-Activating Factor and Related Lipid Mediators 2. Advances in Experimental Medicine and Biology, vol 416. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0179-8_21
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DOI: https://doi.org/10.1007/978-1-4899-0179-8_21
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