Functional Role of Platelet-Activating Factor Receptor in Secretory Response in Adrenal Chromaffin Cells
Platelet-Activating Factor(PAF), a potent bioactive phospholipid mediator in cell-cell communications, is involved in many physiological and also in several pathological processes. The notion that PAF might play an important role in the central nervous system(CNS), particularly in brain ischemia and traumatic injury-induced neuronal damage, was inferred from evidence that a variety of PAF receptor antagonists modified the pathological consequences of brain injury. PAF biosynthesis and degradation were detected in brain as well as its accumulation during ischemia and convulsions. In recent studies, specific biding sites1 and PAF receptor mRNA has been found in CNS2. It has also been shown that PAF increases in intracellular free Ca2+ concentration([Ca2+]i) in PC12 cells3,4, NG108-15 cells5, NCB-206 cells and in rat hippocampal cells2, and stimulates the release of neurotransmitter from PC12 cells3,4, acetylcholine(ACh) release at neuromuscular junction7 and glutamate release from neuronal cells8. On the other hand, PAF inhibits the release of ACh from rat cortex and hippocampus9. PAF also participates in secretory functions of exocrine cells10. We have previously shown that PAF potentiated the stimulation-evoked [Ca2+]i rise and catecholamine(CA) release in adrenal chromaffin cells11. Thus, PAF may be involved in stimulation-secretion coupling.
KeywordsNerve Growth Factor Chromaffin Cell Adrenal Chromaffin Cell 86Rb Uptake Adrenal Medulla Chromaffin Cell
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