Free Radical-Induced Oxidation of Glycerophosphocholine Lipids and Formation of Biologically Active Products
Lipid mediators are a diverse family of biologically active compounds derived from phospholipids and are thought to play important roles both in physiological processes as well as pathophysiologic events. Perhaps the best studied class of lipid mediators are those derived from arachidonic acid, termed eicosanoids. Several enzymatic systems are known to be involved in their formation including prostaglandin H synthase which catalyzes the formation of cyclic endoperoxides, the direct precursor of prostaglandins, thromboxane, and prostacyclinl. Leukotrienes are formed from arachidonic acid by the enzyme 5-lipoxygenase which initially introduces oxygen into the arachidonic acid backbone2. Other biologically active eicosanoids are formed by 15-lipoxygenase3, 12-lipoxygenase4, and cytochrome P-4505. These enzymes use nonesterified arachidonic acid as substrate. Another important class of lipid mediator is platelet-activating factor which is derived from ether phospholipids and has acetate at the sn-2 position. The formation of the precursor lyso glycerophosphocholine as well as the subsequent acetylation process are enzymatically mediated’.
KeywordsArachidonic Acid Lipid Mediator Carbon Centered Radical Ether Phospholipid Washed Rabbit Platelets26
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