Abstract
Delivering therapeutic genes to cells by their complexation with cationic liposomes to form lipoplexes has been shown to be efficient in vitro and in vivo (Behr, 1994; Ledley, 1995). The lipoplexes (plasmid DNA complexed with cationic liposomes) are safer than viral vectors (Mulligan, 1993; Crystal, 1995) for the following reasons: the absence of viral DNA, no constraint on DNA size, protection of DNA from degradation, and ability to target recombinant genes to specific cells. Therefore, lipoplexes might become the mainstream of research for gene therapy. The successful use of these lipoplexes will depend on their efficient delivery to cells and their ability to produce therapeutic levels of gene expression.
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Hirsch-Lerner, D., Zuidam, N.J., Barenholz, Y. (1998). Physicochemical Characterization of DOTAP-Containing Lipoplexes by Fluorescent Probes: Relevance to Lipofection. In: Gregoriadis, G., McCormack, B. (eds) Targeting of Drugs 6. NATO ASI Series, vol 300. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0127-9_16
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