Physicochemical Characterization of DOTAP-Containing Lipoplexes by Fluorescent Probes: Relevance to Lipofection
Delivering therapeutic genes to cells by their complexation with cationic liposomes to form lipoplexes has been shown to be efficient in vitro and in vivo (Behr, 1994; Ledley, 1995). The lipoplexes (plasmid DNA complexed with cationic liposomes) are safer than viral vectors (Mulligan, 1993; Crystal, 1995) for the following reasons: the absence of viral DNA, no constraint on DNA size, protection of DNA from degradation, and ability to target recombinant genes to specific cells. Therefore, lipoplexes might become the mainstream of research for gene therapy. The successful use of these lipoplexes will depend on their efficient delivery to cells and their ability to produce therapeutic levels of gene expression.
KeywordsLipid Bilayer Fluorescence Anisotropy Cationic Lipid Cationic Liposome Static Light Scattering
Unable to display preview. Download preview PDF.
- Barenholz, Y., and Amselem, S., 1993, Quality control assays in the development and clinical use of liposome based formulations, in: Liposome Technology, Vol. 1, 2nd ed., Gregoriadis, G., ed., CRC Press, Boca Raton.Google Scholar
- Chen, W., Carbone, F.R., and McCluskey, J., 1993, Electroporation and commercial liposomes efficiently deliver soluble protein into the MHC class I presentation pathway. Priming in vitro and in vivo for class I-restricted recognation of soluble antigen, J. Immunol. Methods 160:49.PubMedCrossRefGoogle Scholar
- Cullis, P. R., van Dijck, P.W., de Kruijff, B., and de Gier, J., 1978, Effects of cholesterol on the properties of equimolar mixtures of synthetic phosphatidylethanolamine and phosphatidylcholine. A 31P NMR and differential scanning calorimetry study, Biochim. Biophys. Acta 513:21.PubMedCrossRefGoogle Scholar
- Dan, N., 1998, The structure of DNA complexes with cationic liposomes — cylindrical or lamellar? Biochim. Biophys. Acta, in press.Google Scholar
- Feigner, J.H., Kumar, R., Sridhar, S.H., Wheeler, C.J., Feigner, J.H., Tsai, Y.J., Border, R., Ramsey, P., Martin, M., and Felgner, P.L., 1994, Enhanced gene delivery and mechanism studies with novel series of cationic lipid formulations, J. Biol. Chem. 269:2550.Google Scholar
- Felgner, P.L., Tsai, Y.J., and Felgner, J.H., 1996, Advances in the design and application of cytofectin formulations, in: Handbook of Nonmedical Applications of Liposomes, Vol. 4, Chapter 4, Lasic, D.D. and Barenholz, Y., eds., CRC Press, Boca Raton.Google Scholar
- Gruen, D.W.R., Marcelja, S., and Parsegian, V.A., 1984, Water structure near the membrane surface, in: Cell Surface Dynamics: Concepts and Models, Perelson, A.S., DeLisi, C. and Wiegel, F.W., eds., Chapter 3, Marcel Dekker, New York.Google Scholar
- Harned, H.S., and Owen, B.B., 1958, The Physical Chemistry of Electrolytic Solutions, Reinhold, New York.Google Scholar
- Hirsch-Lerner, D., and Barenholz, Y., 1998, Probing DNA-cationic lipid interactions with the fluorophore trimethylammonium diphenyl-hexatriene (TMADPH), Biochim. Biophys. Acta, in press.Google Scholar
- Tatulian, S.A., 1993, in: Phospholipids Handbook, G. Cevc, ed., Marcel Dekker, New York.Google Scholar