Taurine 3 pp 441-447 | Cite as

Increased Phosphorylation of Specific Rat Cardiac and Retinal Proteins in Taurine-Depleted Animals: Isolation and Identification of the Phosphoproteins

  • John B. Lombardini
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 442)


Partial depletion of the tissue levels of taurine in the alive animal stimulates in vitro phosphorylation of both an ~20 kDa (94%) and an ~44 kDa (85%) protein present in subcellular fractions of the rat retina and heart. Tissue levels of taurine were reduced by feeding the animals 1.5% guanidinoethanesulfonic acid (GES) in their drinking water for 6 weeks. The increase in the in vitro phosphorylation of both the ~20 kDa and ~44 kDa proteins was reversed when the animals were subsequently given 1.5% taurine in their drinking water for an additional 6 weeks. Isolation, purification, tryptic digestion, and peptide sequence analysis of the retinal ~20 kDa phosphoprotein suggest that it is histone H2B. However, the results are ambiguous due to an impurity (<25%) in the retinal preparation. Sequence analysis of the ~44 kDa phosphoprotein indicates that it is pyruvate dehydrogenase.


Pyruvate Dehydrogenase Mitochondrial Fraction Approximate Molecular Weight Taurine Level Taurine Content 


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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • John B. Lombardini
    • 1
  1. 1.Departments of Pharmacology and Ophthalmology and Visual SciencesSchool of Medicine, Texas Tech University Health Sciences CenterLubbockUSA

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