Abstract
The effects of taurine and chelerythrine (CHT) on ATP-dependent calcium uptake and the phosphorylation of the ~20 kDa phosphoprotein in the retina were compared. In the absence of the CHT, taurine stimulated ATP-dependent calcium uptake and attenuated the phosphorylation of the ~20 kDa phosphoprotein. On the other hand, CHT produced the opposite results in the absence of taurine. When the two agents were used simultaneously, it was found that CHT non-competitively inhibited the action of taurine to stimulate calcium uptake, while taurine non-competitively inhibited the action of CHT to stimulate the phosphorylation of the ~20 kDa phosphoprotein. The data present an unusual pharmacological mechanism for controlling the signal transduction pathway involving the two distinct cellular processes being studied. Given the unique data, a control system is proposed in which the function of the ~20 kDa phosphoprotein is linked to the stimulation of ATP-dependent calcium uptake.
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Militante, J.D., Lombardini, J.B. (1998). Unique Pharmacological Interactions of Taurine and Chelerythrine in the Retina. In: Schaffer, S., Lombardini, J.B., Huxtable, R.J. (eds) Taurine 3. Advances in Experimental Medicine and Biology, vol 442. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0117-0_53
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DOI: https://doi.org/10.1007/978-1-4899-0117-0_53
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