Taurine 3 pp 247-254 | Cite as

Effects of Steroid Hormones and Cyclosporine A on Taurine-Transporter Activity in the RAW264.7 Cell Line

  • Ha Won Kim
  • Eun Jin Lee
  • Mi Ja Shim
  • Byong Kak Kim
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 442)


The activity of the taurine transporter is affected by various extracellular stimuli, such as ions, hormones and stress. To assess the effects of steroid hormones and cyclosporine A (CsA) on taurine-transporter activity, the murine monocytic cell line, RAW264.7, was stimulated with dexamethasone (DM), triamcinolone (TA), cortisone (CS), hydrocortisone (HCS), prednisone (PSN), prednisolone (PSL) and methylprednisolone (MPSL) in the presence of 12-O-tetradecanoylphorbol 13-acetate (TPA). Treatment of the cell with TPA led to a significant reduction in taurine-transporter activity. However, in the case of the stimulation of the cells with steroid hormones in the presence of TPA, all of the hormones reversed the TPA-induced reduction in the taurine-transporter activity. Treatment of the cells with CsA led to a significant reduction in taurine-transporter activity, but ionomycin (IM) alone did not affect taurine-transporter activity. However, IM reversed the TPA- and CsA-induced reduction in taurine-transporter activity. These results showed that both IM and the glucocorticoid hormones reversed TPA-induced reductions in taurine-transporter activity but only IM reversed the CsA-induced reduction of transporter activity in the RAW264.7 cell line.


Retinal Pigment Epithelium Macrophage Cell Line Glucocorticoid Hormone Human Retinal Pigment Epithelial Cell Taurine Transporter 
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  1. 1.
    Blumberg, P.M., 1988, Protein kinase C as the receptor for the phorbol ester tumor promoters: sixth Rhoads memorial award lecture, Cancer Res., 48:1.PubMedGoogle Scholar
  2. 2.
    Borel, J.F., Seuer, C., Gubler, H.U., and Stahelin, H., 1976, Biological effects of cyclosporin A: a new antilymphocytic agent, Agents Actions, 6:468–475.PubMedCrossRefGoogle Scholar
  3. 3.
    Gordon, R.E., Heller, R.F. and Heller, R.F., 1992, Taurine, Nutritional Value and Mechanisms of Action, pp. 319–328, Plenum Press, New York.Google Scholar
  4. 4.
    Hass, R., Brach, M., Kharbanda, S., Giese, G., Traub, P., and Kufe, D., 1991, Inhibition of phorbol ester-induced monocytic differentiation by dexamethasone is associated with down-regulation of c-fos and c-jun (AP-1), J. Cellular Physiol., 149:125–131.CrossRefGoogle Scholar
  5. 5.
    Jhiang, S.M., Fithian, L., Smanik, P., McGill, J., Tong, Q., and Mazzaferri, E.L., 1993, Cloning of the human taurine transporter and characterization of taurine uptake in thyroid cells, FEBS Letters, 318:139–144.PubMedCrossRefGoogle Scholar
  6. 6.
    Jones, D.P., Miller, L.A., Dowling, C., and Chesney, R.W., 1991, Regulation of taurine transporter activity in LLC-PK1 cells: role of protein synthesis and protein kinase C activation, J. Am. Soc. Nephrol., 2:1021–1029.PubMedGoogle Scholar
  7. 7.
    Kim, H.W., Shim, M.J., Kim, W.B., and Kim, B.K., 1995, Regulation of taurine transporter activity by glucocorticoid hormone, J. Biochem. Mol. Biol., 28:527.Google Scholar
  8. 8.
    Koyama, I., Nakamura, T., Ogasawara, M., Nemoto, M., and Yoshida, T., 1992, Taurine, Nutritional Value and Mechanisms of Action, pp. 355–359, Plenum Press, New York.Google Scholar
  9. 9.
    Kulanthaivel, P., Cool, D.R., Ramamoorthy, S., Mahesh, V.B., Leibach, F.H., and Ganapathy, V., 1991, Tyrosine residues are essential for the activity of the human placental taurine tran-sporter, Biochem. J., 277:53–58.PubMedGoogle Scholar
  10. 10.
    Landewe, R.B., Dijkmans, B.A., and Verdonk, M.J., 1996, Persistent CD3-crosslinking down-regulates interleukin-2 responsiveness in interleukin-2-competent cloned T cells: the possible involvement of protein kinase C, Scand. J. Immunol., 44:45–53.PubMedCrossRefGoogle Scholar
  11. 11.
    Liu, Q.R., Lopez-Corcuera, B., Nelson, H., Mandiyan, S., and Nelson, N., 1992, Cloning and expression of a cDNA encoding the transporter of taurine and β-alanine in mouse brain, Proc. Natl Acad. Sci. USA, 89:12145–12149.PubMedCrossRefGoogle Scholar
  12. 12.
    Miyamoto, Y., Liou, G.I., and Sprinkle, T.J., 1996, Isolation of a cDNA encoding a taurine transporter in the human retinal pigment epithelium, Curr. Eye Res., 15:345–349.PubMedCrossRefGoogle Scholar
  13. 13.
    Munck, L.K. and Munck, B.G., 1992, Distinction between chloride-dependent transport systems for taurine and beta-alanine in rabbit ileum, Am. J. Physiol. 262:G609–615.PubMedGoogle Scholar
  14. 14.
    Oja, S.S. and Kontro, P., 1990, Taurine: Functional Neurochemistry, Physiology and Cardiology, pp. 69–76, Wiley-Liss, New York.Google Scholar
  15. 15.
    Queneau, P.E., Bertault-Peres, P., Mesdjian, E., Durand, A. and Montet, J.C., 1993, Diminution of an acute cyclosporin-induced cholestasis by tauroursodeoxycholate in the rat, Transplant-ation, 56:530–534.CrossRefGoogle Scholar
  16. 16.
    Quinn, M.R. and Miller, C.L., 1992, Taurine allosterically modulates flunitrazepam binding to synaptic membranes, J. Neurosci. Res., 33:136–141.PubMedCrossRefGoogle Scholar
  17. 17.
    Ramamoorthy, S., Del Monte, M.A., Leibach, F.H., and Ganapathy, V., 1994, Molecular identity and calmodulin-mediated regulation of the taurine transporter in a human retinal pigment epithelial cell line, Curr. Eye Res., 13:523–529.PubMedCrossRefGoogle Scholar
  18. 18.
    Ramamoorthy, S., Leibach, F.H., Mahesh, V.B., and Ganapathy, V., 1992, Selective impairment of taurine transport by cyclosporin A in a human placental cell line, Pediatric Res., 32:125–127.CrossRefGoogle Scholar
  19. 19.
    Ramamoorthy, S., Leibach, F.H., Mahesh, V.B., Han, H., Yang-Feng, T., Blakely, R.D., and Ganapathy, V., 1994, Functional characterization and chromosomal localization of a cloned taurine transporter from human placenta, Biochem. J., 300:893–900.PubMedGoogle Scholar
  20. 20.
    Sawamura, A., Azuma, J., Awata, N., Harada, H., and Kishimoto, S., 1990, Taurine: Functional Neurochemistry, Physiology and Cardiology, pp. 207–215, Wiley-Liss, New York.Google Scholar
  21. 21.
    Sivakami, S., Ganapathy, V., Leibach, F.H., and Miyamoto, Y., 1992, The gamma-amino butyric acid transporter and its interaction with taurine in the apical membrane of the bovine retinal pigment epithelium, Biochem. J., 283:391–397.PubMedGoogle Scholar
  22. 22.
    Smith, K.E., Borden, L.A., Wang, C.D., Hartig, P.R., Branchek, T.A., and Weinshank, R.L., 1992, Cloning and expression of a high affinity taurine transporter from rat brain, Mol. Pharmacol., 42:563–569.PubMedGoogle Scholar
  23. 23.
    Tiruppathi, C., Bransdisch, M., Miyamoto, Y., Ganapathy, V., and Leibach, F.H., 1992, Constitutive expression of the taurine transporter in a human colon carcinoma cell line, Am. J. Physiol. 263:G625–631.PubMedGoogle Scholar
  24. 24.
    Uchida, S., Kwon, H.M., Preston, A.S., and Handler, J.S., 1991, Expression of Madin-Darby canine kidney cell Na(+)-and Cl(-)-dependent taurine transporter in Xenopus laevis oocytes, J. Biol. Chem., 266:9605–9609.PubMedGoogle Scholar
  25. 25.
    Uchida, S., Kwon, H.M., Yamauchi, A., Preston, A.S., Marumo, F., and Handler, J.S., 1992, Molecular cloning of the cDNA for an MDCK cell Na+-and Cl—dependent taurine transporter that is regulated by hypertonicity, Proc. Natl. Acad. Sci. USA, 89:8230–8234.PubMedCrossRefGoogle Scholar
  26. 26.
    Vessey, D.A. and Kelley, M., 1995, Inhibition of bile acid conjugation by cyclosporin A, Biochim. Biophys. Acta, 1272:49–52.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Ha Won Kim
    • 1
  • Eun Jin Lee
    • 2
  • Mi Ja Shim
    • 1
  • Byong Kak Kim
    • 2
  1. 1.Department of Life ScienceThe Seoul City University of SeoulSeoulKorea
  2. 2.Department of Microbial ChemistryCollege of Pharmacy, Seoul National UniversitySeoulKorea

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