Advertisement

Taurine 3 pp 177-182 | Cite as

Taurine Chloramine Inhibits the Production of Superoxide Anion, IL-6 and IL-8 in Activated Human Polymorphonuclear Leukocytes

  • Eunkyue Park
  • James Alberti
  • Michael R. Quinn
  • Georgia Schuller-Levis
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 442)

Abstract

Polymorphonuclear leukocytes (PMN) are the initial cells recruited to the site of inflammation where foreign invaders such as microorganisms, toxic gases and chemicals elicit an inflammation reaction4,6,12. Activated PMN produce various oxygen and hydroxyl radicals which kill bacteria and fungi but may also induce indiscriminate cellular damage14. For example, HOCl/OCl- produced by the halide-dependent myeloperoxidase of PMN is highly toxic. Taurine attenuates the damage caused by HOCl/OCl- by forming taurine chloramine (Tau-Cl), a relatively non-toxic and long-lived oxidant7,9,13,15. Previously we demonstrated that Tau-Cl suppressed the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2) in activated murine peritoneal macrophages and/or RAW 264.7, a murine macrophage cell line8,10,11. Tau-Cl also inhibits the production of superoxide anion in activated murine peritoneal PMN5. Since human PMN produce Tau-Cl by halide-dependent myeloperoxidase, we investigated the effect of Tau-Cl on the production of various cytokines and superoxide anion by activated human PMN. In this report we demonstrate that Tau-Cl down-regulates the production of superoxide anion, IL-6 and IL-8 by activated human PMN.

Keywords

Nitric Oxide Superoxide Anion Production Phorbol Myristate Acetate Murine Peritoneal Macrophage Human Polymorphonuclear Leukocyte 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Gordon, R.E., Shaked, A.A., and Solano, D.F., 1986, Taurine protects hamster bronchioles from acute NO2-induced alterations, a histologie, ultrastructural and freeze-fracture study, Am. J. Pathol., 125:585–600.PubMedGoogle Scholar
  2. 2.
    Green T., Fellman, J.H., Eicher, A.I., and Pratt, K.J., 1991, Antioxidant role and subcellular location of hypotaurine and taurine in human neutrophils, Biochem. Biophys. Acta., 1073:93–97.Google Scholar
  3. 3.
    Gurujeyalakshmi, G., Iyer, S.N., Hollinger, M.A., and Geri, S.N., 1996, Procollagen gene expression is down-regulated by taurine and niacin at the transcriptional level in the bleomycin model of lung fibrosis, J. Pharmacol. Exp. Ther., 277:1152–1157.PubMedGoogle Scholar
  4. 4.
    Harada, A., Sekido, N., Akahoshi, T., Wada, T., Mukaida, N., and Matsushima, K., 1994, Essential involvement of interleukin-8 (IL-8) in acute inflammation, J. Leukoc. Biol., 56:559–564.PubMedGoogle Scholar
  5. 5.
    Kim, C., Park, E., Quinn, M.R., and Schuller-Levis, G., 1996, The production of Superoxide anion and nitric oxide by cultured murine leukocytes and the accumulation of TNF-α in the conditioned media is inhibited by taurine chloramine, Immunopharmacology 34:89–95.PubMedCrossRefGoogle Scholar
  6. 6.
    Lloyd, A. and Oppenheim, J., 1992, Poly’s lament: the neglected role of the polymorphonuclear neutrophil in the afferent limb of the immune response, Immunology Today, 13:169–172.PubMedCrossRefGoogle Scholar
  7. 7.
    Marques, L.A. and Dunford, H.B., Chlorination of taurine by myeloperoxidase: kinetic evidence for an enzyme-bound intermediate, J. Biol. Chem., 269:7950-7956.Google Scholar
  8. 8.
    Park, E., Quinn, M.R., Wright, C.E., and Schuller-Levis, G., 1993, Taurine chloramine inhibits the synthesis of nitric oxide and release of tumor necrosis factor in activated RAW 264.7 cells, J. Leukoc. Biol., 54:119–124.PubMedGoogle Scholar
  9. 9.
    Park, E., Schuller-Levis, G., Jia, J., and Quinn, M.R., 1997, Preactivation exposure of RAW 264.7 cells to taurine chloramine attenuates subsequent production of nitric oxide and expression of iNOS mRNA, J. Leukoc. Biol., 61:161–166.PubMedGoogle Scholar
  10. 10.
    Park, E., Schuller-Levis, G., and Quinn, M.R., 1995, Taurine chloramine inhibits production of nitric oxide and TNF-α in activated RAW 264.7 cells by mechanisms that involve transcriptional and translational events, J. Immunol., 154:4778–4784.PubMedGoogle Scholar
  11. 11.
    Quinn, M.R., Park, E., and Schuller-Levis, G., 1996, Taurine chloramine inhibits prostaglandin E2 production in activated RAW 264.7 cells by post-transcriptional effects on inducible cyclooxygenase expression, Immunol. Lett., 50:185–188.PubMedCrossRefGoogle Scholar
  12. 12.
    Schleimer, R., Freeland, H., Peter, S., Brown, K., and Derse, C., 1989, An assessment of the effects of glucocorticoid on degranulation, chemotaxis, binding to vascular endothelium and formation of leukotriene B4 by purified human neutrophils, J. Pharmacol. Exp. Ther., 250:598–602.PubMedGoogle Scholar
  13. 13.
    Schuller-Levis, G., Gordon, R.E., Park, E., Pendino, K., and Laskin, D., 1995, Taurine protects rat bronchioles from acute ozone-induced lung inflammation and hyperplasia, Exp. Lung Res., 21:877–888.PubMedCrossRefGoogle Scholar
  14. 14.
    Weiss, S.J. 1989, Tissue destruction by neutrophils, New Engl. J. Med., 320:365–375.PubMedCrossRefGoogle Scholar
  15. 15.
    Weiss, S.J., Kline, R., Slivka, A., and Wei, M., 1982, Chlorination of taurine by human neutrophils, evidence for hypochlorous acid generation, J. Clin. Invest., 70:598–607.PubMedCrossRefGoogle Scholar
  16. 16.
    Witko, V., Nguyen, A.T., and Descamps-Latscha, B., 1992, Microtiter plate assay for phagocyte-derived taurine chloramine, J. Clin. Lab. Anal., 6:47–53.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Eunkyue Park
    • 1
  • James Alberti
    • 1
  • Michael R. Quinn
    • 2
  • Georgia Schuller-Levis
    • 1
  1. 1.Departments of ImmunologyNY State Institute for Basic Research in Developmental DisabilitiesStaten IslandUSA
  2. 2.Developmental BiochemistryNY State Institute for Basic Research in Developmental DisabilitiesStaten IslandUSA

Personalised recommendations