Taurine 3 pp 105-112 | Cite as

Taurine Protects the Liver against Lipid Peroxidation and Membrane Disintegration during Rat Hepatocarcinogenesis

  • Jeong Soon You
  • Kyung Ja Chang
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 442)


The purpose of this study was to determine the effects of taurine supplementation on both hepatic morphological changes and the extent of hepatic lipid peroxidation and membrane disintegration during rat hepatocarcinogenesis. Sprague Dawley rats were fed high fat diets containing 15% corn oil and were maintained on drinking water with or without 1% taurine. Two weeks after the appropriate feeding regimen, hepatocarcinogenesis was induced by a modification of the Solt and Farber method. This involved a 8 week protocol, including diethylnitrosamine initiation, 3 weeks of 2-acetylaminofluorene feeding and finally a 70% partial hepatectomy. Morphological changes of the hepatocyte were observed by transmission electron microscopy (TEM). Hepatocytes of the carcinogen-treated rat not exposed to taurine contained normal nuclei, but the endoplasmic reticulum (ER) and the mitochondria (Mi) were almost destroyed. By contrast, although the hepatocytes from the taurine supplemented group contained some irregular contour nuclei, the ER and Mi were normal. In the carcinogen-treated groups, lipid peroxidation was decreased because of the activation of several detoxifying enzymes. Glutathione S-transferase (GST) activity increased in the carcinogen-treated groups but less so in the group supplemented with taurine before treatment with the carcinogen. In the group supplemented with taurine prior to treatment with the carcinogen, glutathione peroxidase (GPx) activity was higher than in the carcinogen-treated group lacking taurine exposure. Consistent with the severe destruction to the membrane in the carcinogen-treated rats, hepatic glucose-6-phosphatase (G6Pase) activity, an index of membrane stability, was also decreased. However, both the fall in G6Pase activity and the degree of membrane damage was reduced in the carcinogen-treated animals receiving oral taurine. These results suggest that taurine appears to inhibit lipid peroxidation, to alter the activity of the defense enzymes and to protect the liver against membrane disintegration during rat hepatocarcinogenesis.


Partial Hepatectomy Inhibit Lipid Peroxidation TBARS Content Taurine Supplementation Hepatic Lipid Peroxidation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Alvarez, J.G. and Storey, B.T., 1983, Taurine, hypotaurine, epinephrine and albumin inhibit lipid peroxidation in rabbit spermatozoa and protect against lossofmotility, Biol. Reproduction, 29:548–555.CrossRefGoogle Scholar
  2. 2.
    Aruoma, O.I., Halliwell, B., Honey, B.W., and Butler, J., 1988, The antioxidant action of taurine, hypotaurine and their metabolic precursors, Biochem. J., 256:251–255.PubMedGoogle Scholar
  3. 3.
    Astrom, A., Maner, S., and DePierre, J.W., 1986, Induction of cytochrome p450 and related drug metabolizing activities in the livers of different rodent species by 2-acetylaminoflurorene and 3-methylcholanthrene, Biochem. Pharmacol., 35:2703–2713.PubMedCrossRefGoogle Scholar
  4. 4.
    Azuma, J., Hamaguchi, T., Ohta, H., Takihara, K., Awata, N., Sawamura, A., Harada, H., Tanaka, Y., and Kishimoto, S., 1987, Calcium overload-induced myocardial damage caused by isoproterenol and by adriamycin: possible role of taurine in its prevention, Adv. Exp. Med. Biol., 217:167–179.PubMedGoogle Scholar
  5. 5.
    Baginski, E.S., Foa, P.P., and Zak, B., 1983, Glucose 6-phosphatase, Method. Enz. Anal., 2:876–880.Google Scholar
  6. 6.
    Buege, J.A. and Aust, S.D., 1978, Microsomal lipid peroxidation, Meth. Enzymol., 52:302–310.PubMedCrossRefGoogle Scholar
  7. 7.
    Fleisher, S. and Kervina, M., 1974, Subcellular fraction of rat liver, Meth. Enzymol., 31:6–13.CrossRefGoogle Scholar
  8. 8.
    Gordon, R.E., Richard, F.H., and Rachel, F.H., 1992, Taurine protection of lungs in hamster weeks of oxidant injury: a morphologic time study of paraquat and bleomycin treatment, Adv. Exp. Med. Biol., 315:319–328.PubMedCrossRefGoogle Scholar
  9. 9.
    Habig, W.H., Pabst, M.J., and Jakoby, W.B., 1974, Glutathione S-transferase, J. Biol. Chem., 249:7130–7139.PubMedGoogle Scholar
  10. 10.
    Howie, A.F., Forrester, L.M., Glancey, M.J., Schlager, J.J., Powis, G., Beckett, G.J., Hayes, J.D., and Wolf, C.R., 1990, Glutathione S-transferase and glutathione peroxidase expression in normal and tumor human tissues, Carcinogenesis, 11:451–458.PubMedCrossRefGoogle Scholar
  11. 11.
    Huxtable, R.J., 1992, Physiological actions of taurine, Physiol. Rev., 72:101–163.PubMedGoogle Scholar
  12. 12.
    Kulakowski, E.C. and Matura, J., 1984, Hypoglycemic properties of taurine: not mediated by enhanced insulin release, Biochem. Pharmac., 33:2835–2838.CrossRefGoogle Scholar
  13. 13.
    Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J., 1951, Protein measurement with Folin phenol reagent, J. Biol. Chem., 193:265–275.PubMedGoogle Scholar
  14. 14.
    Mukai, F.H., Goldstein, B.D., 1981, Mutagenicity of malondialdehyde, a decomposition product of peroxidized polyunsaturated fatty acids, Science, 197:868–869.Google Scholar
  15. 15.
    Nakashima, T., Seto, Y., Toshikazu, N., Shima, T., Iwai, Y., Zeiao, K., Okanque, T., and Kashima, K., 1990, Calcium-associated cytoprotective effect of taurine on the calcium and oxygen paradoxes in isolated rat hepatocytes, Liver, 10:167–172.PubMedGoogle Scholar
  16. 16.
    Nakashima, T., Takino, T., and Kuriyama, K., 1982, Therapeutic effect of taurine administration on carbon tetrachloride-induced hepatic injury, Japan J. Pharm., 32:583–589.CrossRefGoogle Scholar
  17. 17.
    Nakashima, T., Takino, T., and Kuriyama, K., 1983, Pretreatment with taurine facilitates hepatic lipid peroxide formation associated with carbon tetrachloride intoxidation, Japan J. Pharm., 33:515–523.CrossRefGoogle Scholar
  18. 18.
    Park, K.A., 1994, Effect of different fats and proteins on the hepatic lipid peroxidation and membrane stability in phenobarbital-induced hepatocarcinogenesis-Casein vs Soy protein-, Master thesis of Seoul National University.Google Scholar
  19. 19.
    Pasantes-Morales, H. and Cruz, C., 1985, Taurine and hypotaurine inhibit light-induced lipid peroxidation and protect rod outer segment structure, Brain Res., 330:154–157.PubMedCrossRefGoogle Scholar
  20. 20.
    Solt, D. and Farber, E., 1976, New principle for the analysis of chemical carcinogenesis, Nature, 263:701–703.CrossRefGoogle Scholar
  21. 21.
    Stout, D.L. and Becker, F.F., 1986, Xenobiotic metabolizing enzymes in genetically and chemically initiated mouse liver tumors, Cancer Res., 46:2693–2696.PubMedGoogle Scholar
  22. 22.
    Tappel, A.L., 1978, Glutathione peroxidase and hydroperoxides, Meth. Enzymol., 52:506–513.PubMedCrossRefGoogle Scholar
  23. 23.
    Zhou, M., Ma, T., and Tseng, M.T., 1991, Effects of taurine and ketamine on bovine retinal membrane lipid peroxidation, Neuroscience, 45(2):461–465.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Jeong Soon You
    • 1
  • Kyung Ja Chang
    • 1
  1. 1.Department of Food and NutritionInha UniversityInchonKorea

Personalised recommendations