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Effects on Female Fertility and Germinal Cells in Prepubertal and Adult Rats (Rattus norvegicus) after X-Ray Irradiation

  • Ivan Martínez-Flores
  • Josep Egozcue
  • Montserrat Garcia
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 444)

Abstract

In this work we analise the effects of ionizing radiation in prepubertal and adult female rats, using as parameters, implantation sites, embryonic loss, and synaptonemal complex (SC) analysis of the female F1 of the pregnant rats. Our preliminary results show a decrease statisticaly significant in fertility of irradiated groups, but cytogenetic analysis did not shown any inherited damage atributed to radiation. At the same time, our results seem to indicate a higher resistance of oocytes from prepubertal rats and support the possible use of GnRH agonists as oestrus cycle supressors to turn adult rats into gonadal quiescence.

Keywords

GnRH Agonist Synaptonemal Complex Mouse Oocyte Irradiate Group Embryonic Loss 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Brewen, J.G., Payne, H.S., Preston, R.J., 1976, X-ray-induced chromosome aberrations in mouse dictyate oocytes by X-rays. I. Time and dose relationships. Mutation Res, 35: 111–120.PubMedCrossRefGoogle Scholar
  2. Brewen, J.G., Payne, H.S., 1979, X-ray stage sensitivity of mouse oocytes and its bearing dose-response curves. Genetics, 91: 149–161.PubMedGoogle Scholar
  3. Cox, B.D., Lyon, M.F., 1975, X-ray-induced dominant lethal mutations in mature and immature oocytes of guinea pigs and golden hamsters. Mutation Res., 28: 421–436.PubMedCrossRefGoogle Scholar
  4. Jarrell, J., Young Lai, E.V., Barr, R., McMahon, A., Belbeck, L., O’Connell, G., 1986, An analysis of the effects of increasing doses of ionizing radiation to the exteriorized rat ovary on follicular development, atresia and serum gonadotropin levels. Am. J. Obstet. Gynecol, 154: 306–309.PubMedGoogle Scholar
  5. Johannisson, R., Mörmel, R., Brandenburg, B., 1994, Synaptonemal complex damage in fetal mouse oocytes induced by ionising irradiation. Mutation Res., 311: 319–328.PubMedCrossRefGoogle Scholar
  6. Pujol, R., Cusidó, L., Rubio, A., Egozcue, J., Garcia, M., 1996, Effect of X-rays on germ cells in female fetuses of Rattus norvegicus irradiated at three different times of gestation. Mutation Res. 356: 247–253.PubMedCrossRefGoogle Scholar
  7. Pujol, R., Cusidó, L., Rubio, A., Egozcue, J., Garcia, M., 1997, X-ray-induced synaptonemal complex damage during meiotic prophase in female fetuses of Rattus norvegicus. Mutation Res. 379: 127–134PubMedCrossRefGoogle Scholar
  8. Reichert, W., Buselmaier, W., Vogel, F., 1984, Elimination of X-ray induced chromosomal aberrations in the progeny of female mica. Mutation Res. 139: 87–94.PubMedCrossRefGoogle Scholar
  9. Tateno, H., Mikamo, K., 1989, Effects of neonatal ovarian X-irradiation in the Chinese hamster. II. Absence of chromosomal and developmental damages in surviving oocytes irradiated at the pachytene and resting dictyate stages. J. Radiat. Res. 30: 209–217.PubMedCrossRefGoogle Scholar
  10. Tease, C., 1982, Radiation-induced chromosome nondisjunction in oocytes stimulated by different doses of superovulating hormones, Mutation Res., 105: 95–100.PubMedCrossRefGoogle Scholar
  11. Tease, C., 1985, Dose related chromosome non-disjunction in female mice after X-irradiation of dictyate oocytes. Mutation Res. 151: 109–119.PubMedCrossRefGoogle Scholar
  12. Tease, C., Fisher, G., 1991, The influence of maternal age on radiation-induced chromosome aberrations in mouse oocytes. Mutation Res. 262: 57–62.PubMedCrossRefGoogle Scholar
  13. Tease, C., Fisher, G., 1996a, Cytogenetic and genetic studies of radiation induced chromosome damage in mouse oocytes. I. Numerical and structural chromosome anomalies in metaphase II oocytes, pre-and post-implantational embryos. Mutation Res. 349: 145–153.PubMedCrossRefGoogle Scholar
  14. Tease, C., Fisher, G., 1996b, Cytogenetic and genetic studies of radiation induced chromosome damage in mouse oocytes. II. Induced crhomosome loss and dominant visible mutations. Mutation Res. 349: 155–1162.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Ivan Martínez-Flores
    • 1
  • Josep Egozcue
    • 1
  • Montserrat Garcia
    • 1
  1. 1.Departament de Biologia Cellular i Fisiologia., Unitat de Biologia Facultad de MedicinaUniversitat Autónoma de BarcelonaBellaterraSpain

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