Current and Future Contributions of Transgenic Mice to the Analysis of Germline Toxicology
Part of the
Advances in Experimental Medicine and Biology
book series (AEMB, volume 444)
Evermore sophisticated tests are need to study germline toxicology. The gene conversion-based systems developed in Leicester and in the USA are steps in the right direction, but a lot of validation both in vivo and in vitro is required. Transgenic technology can also be used to research the biology of testis, so that we know more how to make it more human-like. If you talk to toxicologists, they always complain: ‘but it’s only a rat, it’s only a mouse, it’s not a man’. In future, once we understand more biology - it might be possible to make the toxicological response of a transgenic mouse more human-like. As we all know, the testis is a complex biological system and it is only when we get a better understanding of what is going on to the fundamental level are such developments possible. Indeed, it might be possible to do even more exciting things, such as taking mitotic human tissue culture cells and to inducing them to enter meiosis in vitro. Such a system would be a natural complement to the in vitro tests widely used in industry.
KeywordsTransgenic Mouse Sertoli Cell Gene Conversion Meiotic Recombination Gene Conversion Event
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Jeffreys, A.J. et al.
, 1994, Complex gene conversion events in germline mutation at human minisatellites, Nature Genetics
6, 136–145.PubMedCrossRefGoogle Scholar
Jeffreys, A.J. and Neumann, R., 1997, Somatic mutation processes at a human minisatellite, Human Molecular Genetics
6, 129–136.PubMedCrossRefGoogle Scholar
Buard, J. and Vergnaud, G., 1994, Complex recombination events at the human minisatellite CEB1 (D2S90), EMBO journal
13: 3203–3210.PubMedGoogle Scholar
Dubrova, Y.E., Jeffreys, A.J. and Malashenko, A.M., 1993, Mouse minisatellite mutations induced by ionizing-radiation, Nature Genetics
5, 92–94.PubMedCrossRefGoogle Scholar
Sadamoto, S. et al.
, 1994, Radiation induction of germline mutation at a hypervariable mouse minisatellite locus, International J. Radiation Biol
65, 549–557.CrossRefGoogle Scholar
Dubrova, Y. et al.
, 1996, Human minisatellite mutation rate after the Chernobyl accident Nature
380, 683–686.PubMedCrossRefGoogle Scholar
Bois, P., Collide, A., Brown J. and Jeffreys, A.J., 1997, Human minisatellite MS32 (D1S8) display somatic but not germline instability in transgenic mice, Human Molecular Genetics
6, 1565–1571.PubMedCrossRefGoogle Scholar
Murti, R., Schimenti K.J. and Schimenti, J.C., 1994, A recombination based transgenic mouse system for genotoxicity testing, Mutation Research
307, 583–595.PubMedCrossRefGoogle Scholar
Akgun, E. et al.
, 1997, Palindrome resolution and recombination in the mammalian germ line, Molecular and Cellular Biology
17, 5559–5570.PubMedGoogle Scholar
Hanneman, W.H., Schimenti K.J. and Schimenti, J.C., 1997, Molecular analysis of gene conversion in spermatids from transgenic mice, Gene
200, 185–192.PubMedCrossRefGoogle Scholar
Bradley, A. and Liu, P., 1996, Target practice in transgenics Nature Genetics
14, 121–123.PubMedCrossRefGoogle Scholar
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