Protection Against Tuberculosis by Plasmid DNA
The availability of cloned mycobacterial genes and suitable vectors for expression in mammalian cells has now opened a new avenue in which individual mycobacterial protein antigens can be tested for their ability to confer protective immunity. Expression of protection against mycobacteria is largely a function of antigen specific CD4 and cytotoxic CD8 T cells. We have found that intramuscular injection of plasmid DNA expressing several mycobacterial immunodominant genes can induce both T cell populations and confer protection equivalent to BCG, suggesting that this approach may lead to a new vaccine.
KeywordsHsp65 Gene Tuberculosis H37Rv Mycobacterial Antigen Immune Spleen Cell Mycobacterial Hsp65
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- Freeman, G. J., Gray, G. S., Gimmi, C. D., Lombard, D. B., Zou, L. White, M., Fingeroth, J. D., Gribben, J. G., and Nadler, L. M., 1991, Structure, expression, and T cell costimulatory activity of the murine homologue of the human B lymphocyte activation antigen B7. J. Exp. Med., 174: 625.PubMedCrossRefGoogle Scholar
- Silva, C.L. and Lowrie, D.B., 1994, A single mycobacterial protein (hsp65) expressed by a transgenic antigen-resenting cell vaccinates mice against tuberculosis. Immunol., 82: 244.Google Scholar
- Silva, C.L., Silva, M.F., Pietro, R.C.L.R. and Lowrie, D.B. 1994, Protection against tuberculosis by passive transfer with T-cell clones recognizing mycobacterial heat-shock protein 65. Immunol., 83: 341.Google Scholar