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Biochemical Properties of Alcohol Dehydrogenase and Glutamate Dehydrogenase Encapsulated into Human Erythrocytes by a Hypotonic-Dialysis Procedure

  • Silvia Sanz
  • Carmen Lizano
  • Marina I. Garín
  • José Luque
  • Montserrat Pinilla

Summary

The stability against time (up to 170h) of encapsulated enzymes were studied in ADH- and GDH-carrier RBCs, at 4ºC and 37ºC, in comparison with that of free enzyme solutions. Encapsulation into RBCs suggest a protective effect of both enzyme activities. An efflux of the encapsulated enzymes from the carrier RBCs was observed during a similar incubation. The continuous degradation of ethanol and the simultaneous appearance/disappearance of acetaldehyde by ADH-RBCs, as a function of time (up to 72h), suggest the use of these carrier RBCs to fully metabolize ethanol. The rapid utilization of ammonia in the presence of GDH-RBCs suggest the use of these RBCs as carrier systems. These properties open the possibility of using ADH- and GDH-RBCs as carrier systems under in vivo situations.

Keywords

Alcohol Dehydrogenase Glutamate Dehydrogenase Mean Cell Volume Human RBCs Index Human Blood 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Silvia Sanz
    • 1
  • Carmen Lizano
    • 1
  • Marina I. Garín
    • 1
  • José Luque
    • 1
  • Montserrat Pinilla
    • 1
  1. 1.Dpto. Bioquimica y Biol. Mol.Univ. AlcalaAlcala de Henares, MadridSpain

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