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Role of Environmental Factors in Regulating Nitrate Respiration in Intertidal Sediments

  • Rodney A. Herbert
  • David B. Nedwell
Part of the Federation of European Microbiological Societies Symposium Series book series (FEMS, volume 56)

Abstract

The microbial decomposition of moribund organic matter involves the consumption of an equivalent quantity of mineral oxidant, either directly as in the case of respiratory metabolism, or indirectly as a consequence of fermentation. Oxidants used in respiratory metabolism include O2, NO 3 and NO 2 , manganese and ferric oxides, SO 4 and CO2. Organic matter decomposition within sediments results in the depletion of these oxidants. As a consequence microbial processes which take place in the surface layers of sediments are not only intimately related to the quality and quantity of organic carbon present but to the concentration and availability of potential inorganic electron acceptors. Aerobic respiration, which takes place in the surface layers of sediments, results in the rapid depletion of 02 and alternative electron acceptors, if present, are then successively utilised by different physiological groups of microorganisms thus creating more reduced conditions (Kaplan, 1979). In the absence of 02 many aerobic and facultatively anaerobic bacteria are able to carry out NO 3 respiration using NO 3 as terminal electron acceptor (Payne, 1973; Herbert, 1982). In NO 3 respiration the end-product(s) of reduction depend (Abd. Aziz and Nedwell, 1986) upon the microorganism involved, growth conditions and may be either NO 2 , NH 4 + , N2O or N2. Whilst NO 3 respiration to NO 2 is a fairly widespread property of chemosynthetic bacteria the further reduction of NO 2 is carried out by fewer species. When the end-products are gaseous the process is termed denitrification and this represents a net loss of nitrogen from an ecosystem. In contrast a number of fermentative bacteria have been shown to reduce NO 2 to NH 4 + with the resultant conservation of nitrogen in a potentially useful form (Hadjepetrou and Stouthamer, 1965; Dunn et al., 1978; Cole and Brown, 1982; Macfarlane and Herbert, 1982). This process termed ‘NO 3 ammonification’ has been shown to occur widely in freshwater, estuarine and marine environments.

Keywords

Overlie Water Nitrate Reduction Estuarine Sediment Paracoccus Denitrificans Fermentative Bacterium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Abd. Aziz, A.S., and Nedwell, D.B., 1986, The Nitrogen Cycle of an East Coast, U.K. Saltmarsh: II Nitrogen Fixation, Nitrification, Denitrification, Tidal Exchange, Estuar. Coastal Shelf Sci. 22, 689.CrossRefGoogle Scholar
  2. Billen, G., 1982, Modelling the processes of organic matter degradation and nutrient recycling in sedimentary systems, In: “Sediment Microbiology,” pp. 1–52, D.B. Nedwell and C.M. Brown, eds., Academic Press, London.Google Scholar
  3. Cole, J.A., and Brown, C.M. 1982, Nitrate reduction to ammonia by fermentative bacteria, FEMS Microbiol Lett., 7: 65.CrossRefGoogle Scholar
  4. Dunn, G.M., Herbert, R.A., and Brown, C.M., 1978, Physiology of denitrifying bacteria from tidal mudflats in the River Tay, In: “Physiology and Behaviour of Marine Organisms,” pp. 135–140Google Scholar
  5. D.S. McLusky and A.J. Berry, eds., Pergamon Press, Oxford.Google Scholar
  6. Dunn, G.M., Wardell, J.M., Herbert, R.A., and Brown, C.M., 1980, Enrichment, enumeration and characterisation of nitrate reducing bacteria present in sediments of the River Tay estuary, Proc. Roy. Soc. Edin. 78B, 47.Google Scholar
  7. Evans, C.G.T., Herbert, D., and Tempest, D.W., 1970, The continuous culture of microorganisms, In: “Methods of Microbiology,” Vol. 2, pp. 277–327, Academic Press, London.Google Scholar
  8. Hadjepetrou, L.P., and Stouthamer, A.H., 1965, Energy production during nitrate respiration by Aerobacter aerogenes, J. Gen. Microbiol. 38, 29.Google Scholar
  9. Herbert, R.A., 1982, Nitrate dissimilation in marine and estuarine sediments, In: “Sediment Microbiology,” pp. 53–71, D.B. Nedwell and C.M. Brown, eds., Academic Press, London.Google Scholar
  10. Jannasch, H.W., 1967, Enrichments of aquatic bacteria in continuous culture, Arch. Mikrobiol. 59, 165.Google Scholar
  11. Jorgensen, K.S. and J. Sorensen, 1988, Two annual maxima of nitrate reduction and denitrification in estuarine sediment (Norsminde fjord, Denmark). Mar. Ecol. Pros. Ser. 48: 147–154.CrossRefGoogle Scholar
  12. Kaplan, W.A., Valiela, I., and Teal, J.M., 1979, Denitrification in a marsh ecosystem, Limnol. Oceanogr. 24, 726.Google Scholar
  13. Keith, S.M., Russ, M.A., Macfarlane, G.T., and Herbert, R.A., 1987, The ecology and physiology of anaerobic bacteria isolated from Tay estuary sediments. Proc. Roy. Soc. Edin. 92B, 323.Google Scholar
  14. King, D., and Nedwell, D.B., 1984, Changes in the nitrate reducing community of an anerobic saltmarsh sediment in response to seasonal selection by temperature J. Gen. Microbiol 130, 2935.Google Scholar
  15. King, D., and Nedwell, D.B., 1985, The influence of nitrate concentration upon the endproducts of nitrate dissimilation by bacteria in anaerobic salt marsh sediments, FEMS Microbiol. Lett. 31, 23.CrossRefGoogle Scholar
  16. King, D., and Nedwell, D.B., 1987, The adaptation of nitrate reducing bacterial communities in estuarine sediments in response to overlying nitrate load, FEMS Microb. Ecol. 45, 15.CrossRefGoogle Scholar
  17. Koike, I., and Hattori, A., 1978, Denitrification and ammonia formation in anaerobic coastal sediments, Appl. Environ. Microbiol. 35, 278.Google Scholar
  18. Koike, I., and Sorensen, J., 1988, Nitrate reduction and denitrification in marine sediments, In: “Nitrogen cycling in coastal marine environments,” T.H. Blackburn and J. Sorensen, eds., pp. 251–273, J. Wiley and Sons, Chichester.Google Scholar
  19. Macfarlane, G.T., and Herbert, R.A., 1982, Nitrate dissimilation by Vibrio spp isolated from estuarine sediments, J. Gen. Microbiol. 128, 2463.Google Scholar
  20. Macfarlane, G.T., and Herbert, R.A., 1984, Dissimilatory nitrate reduction and nitrification in estuarine sediments J. Gen.Microbiol. 130, 2301.Google Scholar
  21. Nedwell, D.B., 1975, Inorganic nitrogen metabolism in a eutrophicated tropical mangrove estuary., Wat. Res. 9, 221.Google Scholar
  22. Nedwell, D.B., 1982, Exchange in nitrate and the products of nitrate reduction between seawater and sediment from a U.K. salt marsh, Estuar. Coastal Shelf Sci. 14, 557.CrossRefGoogle Scholar
  23. Nedwell, D.B., Hall, S.E., Andersson, A., Hagstrom, A., and Lindstrom, E.B., 1983, Seasonal changes in the distribution and exchange of inorganic nitrogen between sediments and water in the northern Baltic (Gulf of Bothnia), Estuar. Coastal Shelf Sci. 17, 169.Google Scholar
  24. Oremland, R.S., Umberger, C., Culbertson, C.W., and Smith, R.L., 1984, Denitrification in San Francisco sediments, Appl. Environ. Microbiol. 47, 1106.Google Scholar
  25. Payne, W.J., 1973, Reduction of nitrogenous oxides by microorganisms, Bact. Rev. 37, 409.PubMedGoogle Scholar
  26. Rehr, B., and Klemme, J.-H., 1989, Competition for nitrate between denitrifying Pseudomonas stutzeri and nitrate ammonifying enterobacteria. FEMS Microbiol. Ecol. 62, 51.Google Scholar
  27. Samuelson, M.O., 1985, Dissimilatory nitrate reduction to nitrite, nitrous oxide and ammonium by Pseudomonas putrefaciens, Appl. Environ. Microbiol. 50, 812.Google Scholar
  28. Samuelson, M.O. and Ronner, U. 1982, Ammonium production by dissimilatory nitrate reducers. Appl. Environ. Microbiol. 44, 1241.Google Scholar
  29. Samuelson, M.O., Cadez, P., and Gustafsson, L. 1988, Heat production by the denitrifying bacterium, Pseudomonas fluorescens and the dissimilatory ammonium producing bacterium Pseudomonas putrefaciens during anaerobic growth with nitrate as electron acceptor, Appl. Environ. Microbiol.,54 2220–2225.Google Scholar
  30. Smith, M., 1982, Dissimilatory nitrate reduction of NO2 - to NH4’ and N20 by a soil citrobacter sp., Appl. Environ. Microbiol. 43, 854.Google Scholar
  31. Smith, C.J., De Laune, R.D., and Patrick, W.H.Jr., 1982, Nitrate reduction in Spartina alterniflora marsh soil. Soil. Sci. Soc. Am. J., 46, 748–750.Google Scholar
  32. Sorensen J., 1978, Capacity for denitrification and reduction of nitrate to ammonia in a coastal marine sediment Appl. Environ. Microbiol. 35, 301.Google Scholar
  33. van Kessel, J.F., 1978, Gas production in aquatic sediments in the presence and absence of nitrate. Water Res. 12, 291.Google Scholar
  34. Wheatland, A.B., Barnett, M.J., and Bruce, A.M., 1959, Some observations of denitrification in rivers and estuaries. Journal of the Institute of Sewage Purification 2, 149.Google Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Rodney A. Herbert
    • 1
  • David B. Nedwell
    • 2
  1. 1.Department of Biological SciencesUniversity of DundeeDundeeUK
  2. 2.Department of BiologyUniversity of EssexColchesterUK

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