Constructing Artificial Antigen-Presenting Cells from Drosophila Cells
Stimulation of unprimed T cells is controlled by professional antigen-presenting cells (APC) such as dendritic cells (DC).1–3 The strong APC function of DC is presumed to reflect that these cells express a high density of major histocompatibility complex (MHC) molecules and a variety of costimulatory molecules. In this respect, activation of naive T cells by APC is thought to require two distinct signals: Signal t reflects T cell receptor (TCR) contact with specific peptides bound to MHC molecules, and Signal 2 is the consequence of other molecules on T cells, e.g. CD28, interacting with costimulatory molecules, e.g. B7 (B7-1, B7-2), on APC. Since many different accessory molecules on APC can express costimulatory function for T cells under defined conditions, which particular accessory molecules are essential for stimulating naive T cells is unclear. We have addressed this issue by constructing artificial APC from a Drosophila cell line by gene transfection.
KeywordsMajor Histocompatibility Complex Major Histocompatibility Complex Class Proliferative Response Costimulatory Molecule Drosophila Cell
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