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The Regulation of T Cell Responses by a Subpopulation of CD8+DEC205+ Murine Dendritic Cells

  • Vadim Kronin
  • Gabriele Süss
  • Ken Winkel
  • Ken Shortman
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 417)

Abstract

Dendritic cells (DC) are migratory bone-marrow-derived cells of sparse but broad tissue distribution1,2. They are not homogeneous and even within a particular lymphoid organ distinct subpopulations can be separated3–5. If procedures are used which extract all DC, both CD8α+ and CD8α DC can be isolated from mouse spleen5. Under these conditions the DC isolated from thymus are predominantly CD8+, whereas those isolated from lymph nodes (LN) are predominantly CD8. This heterogeneity may be of functional importance, since there is evidence that the CD8+ DC of the thymus are of different developmental origin from the classical CD8 DC typically found in LW6,7. Since conventional isolation procedures yielded mainly the CD8 DC population the functional capacity of CD8+ DC had not previously been explored. To determine whether CD8+ and CD8 splenic DC differed in function, we tested their capacity to stimulate purified allogeneic CD4 and CD8 T cells in a primary mixed leucocyte reaction.

Keywords

Dendritic Cell Proliferative Response Mouse Spleen Dendritic Cell Population Murine Dendritic Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Vadim Kronin
    • 1
  • Gabriele Süss
    • 1
  • Ken Winkel
    • 1
  • Ken Shortman
    • 1
  1. 1.The Walter and Eliza Hall Institute of Medical ResearchMelbourneAustralia

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