CD34+ Hematopoietic Progenitors from Human Cord Blood Differentiate Along two Independent Dendritic Cell Pathways in Response to GM-CSF+TNFα
DC are professional antigen presenting cells which are required for the initiation of immune responses. Many types of DC with subtle differences in phenotype have been described in peripheral blood, skin and lymphoid organs (1–8). Although each of these DC subsets display the ability to activate naive T cells, it is not clear whether they represent different stages of maturation of a unique DC lineage or whether they stem from different progenitors. In this study we demonstrate that in presence of GM-CSF+TNFα i) human myeloid progenitors can differentiate along two unrelated DC pathways: the Langerhans cells (LC) characterized by the expression of CD la, Lag, Birbeck granules (BG) and E cadherin and a CD14-derived DC, related to monocyte-derived DC, characterized by the expression of CD la, CD9, CD2 and factor XIIIa. ii) These two subsets display properties expected for DC, but in cocultures with CD40-activated naive B cells only the CD 14+ derived DC can induced the production of IgM in presence of IL-2. These results suggest that the CD14-derived DC type might be preferentially involved in development of humoral responses, while both populations can induce T cell priming.
KeywordsDendritic Cell Human Immunodeficiency Virus Type Hematopoietic Progenitor Factor XIIIa Professional Antigen Present Cell
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