The TH1 Lymphokine Interferon-γ is a Potent Upregulator of Dendritic Cells with Phagocytic Capacity in GM-CSF Supplemented Bone Marrow Cultures

  • Christoph Scheicher
  • Heike Corban
  • Vera Hof
  • Michael Robbers
  • Konrad Reske
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 417)


Myeloid dendritic cells (DC), macrophages and granulocytes are descendants of a hematopoietic progenitor cell that originates in the bone marrow1. Thus, bone marrow derived cells distributed in tissue culture in the presence of GM-CSF give rise to the three leukocyte populations which under various in vitro culture conditions proceed in differentiation and phenotypic maturation2–7.


Dendritic Cell Cutaneous Leishmaniasis Mouse Bone Marrow Bone Marrow Culture Phagocytic Capacity 
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  1. 1.
    K. Inaba, M. Inaba, M. Deguchi, K. Hagi, R. Yasumizu, S. Ikehara, S. Muramatsu, and R. M. Steinman, Granulocytes, makrophages, and dendritic cells arise from a common major histocompatibility complex class II-negative progenitor in mouse bone marrow. Proc. Natl. Acad. Sci. USA 90: 3038–3042 (1993).PubMedCrossRefGoogle Scholar
  2. 2.
    K. Inaba, R. M. Steinman, M. Witmer Pack, H. Aya, M. Inaba, T. Sudo, S. Wolpe, and G. Schuler, Identification of proliferating dendritic cell precursors in mouse blood. J. Exp.Med. 175: 1157–1167 (1992).PubMedCrossRefGoogle Scholar
  3. 3.
    C. Scheicher, M. Mehlig, R. Zecher and K. Reske, Dendritic cells from mouse bone marrow: in vitro differentiation using low doses of recombinant granulocyte-macrophage colony-stimulating factor. J. Immunol. Meth. 154: 253–264 (1992).CrossRefGoogle Scholar
  4. 4.
    K. Inaba, M. Inaba, N. Romani, H. Aya, M. Deguchi, S. lkehara, S. Muramatsu, and R. M. Steinman, Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor. J. Exp. Med. 176: 1693–1702 (1992).PubMedCrossRefGoogle Scholar
  5. 5.
    C. Caux, C. Dezutter-Dambuyant, D. Schmitt and J. Banchereau, GM-CSF and TNF-a cooperate in the generation of dendritic Langerhans cells. Nature 360: 258–261 (1992).PubMedCrossRefGoogle Scholar
  6. 6.
    C. Reid, A. Stackpoole, A. Meager, and J. Tikerpae, Interactions of tumor necrosis factor with granulocyte-macrophage colony-stimulating factor and other cytokines in the regulation of dendritic cell growth in vitro from early bipotent CD34* progenitors in human bone marrow. J. Immunol. 149: 2681–2688 (1992).PubMedGoogle Scholar
  7. 7.
    F. Sallusto and A. Lanzavecchia, Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor a. J. Exp. Med. 179: 1109–1118 (1994).PubMedCrossRefGoogle Scholar
  8. 8.
    K. Inaba, M. Inaba, M. Naito, and R. M. Steinman, Dendritic cell progenitors phagocytose particulates, including Bacillus Calmette-Guerin organisms, and sensitize mice to mycobacterial antigens in vivo. J. Exp. Med. 178: 479–488 (1993).PubMedCrossRefGoogle Scholar
  9. 9.
    C. Reis e Sousa, P. D. Stahl, and J. M. Austyn, Phagocytosis of antigens by Langerhans cells in vitro. J. Exp. Med. 178: 509–519 (1993).CrossRefGoogle Scholar
  10. 10.
    C. Blank, H. Fuchs, K. Rappersberger, M. Röllinghoff, and H. Moll, Parasitism of epidermal Langerhans cells in experimental cutaneous leishmaniasis with Leishmania major. J. Infect. Dis. 167: 418–425 (1993).PubMedCrossRefGoogle Scholar
  11. 11.
    C. Scheicher, M. Mehlig, H.-P. Dienes and K. Reske, Uptake of microparticle-adsorbed protein antigen by bone marrow-derived dendritic cells results in up-regulation of interleukin-la and interleukin-12 p40/p35 and triggers prolonged, efficient antigen presentation. Eur. J. Immunol. 25: 1566–1572 (1995).PubMedCrossRefGoogle Scholar
  12. 12.
    S. E. Macatonia, C.-S. Hsieh, K. M. Murphy, and A. O’Garra, Dendritic cells and macrophages are required for Thl development of CD4’ T cells from a(3 TCR transgenic mice: IL-12 substitution for macrophages to stimulate IFN-y production is IFN-y-dependent. Internat. Immunol. 5: 1119–1128 (1993).CrossRefGoogle Scholar
  13. 13.
    J. W. Kappler, B. Skidmore, J. White, P. Marrack, Antigen-inducible, interleukin-2-producing T cell hybridomas. Lack of independent antigen and H-2 recognition. J. Exp. Med. 153: 1198–1214 (1981).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Christoph Scheicher
    • 1
  • Heike Corban
    • 1
  • Vera Hof
    • 1
  • Michael Robbers
    • 1
  • Konrad Reske
    • 1
  1. 1.Institut für ImmunologieJohannes Gutenberg Universität MainzMainzGermany

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