Abstract
Efficient methods to generate large numbers of dendritic cells have been developed in the past five years. Caux et al.1 have introduced the approach to grow dendritic cells from rare CD34+ progenitor cells in (cord) blood using GM-CSF and TNF-α as the critical cytokines. On the other hand, Sallusto et al.2 and Romani et al.3 have established procedures that make use of the more abundant monocytic CD34-negative and CD14+precursors in peripheral blood. GM-CSF and IL-4 were the necessary cytokines. Both approaches have since been widely used, even up to the stage of clinical trials.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Caux, C., C. Dezutter-Dambuyant, D. Schmitt, and J. Banchereau. 1992. GM-CSF and TNF-a cooperate in the generation of dendritic Langerhans cells. Nature 360: 258–261.
Sallusto, F. and A. Lanzavecchia. 1994. Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor a. J. Exp. Med. 179: 1109–1118.
Romani, N., S. Gruner, D. Brang, E. Kämpgen, A. Lenz, B. Trockenbacher, G. Konwalinka, P. O. Fritsch, R. M. Steinman, and G. Schuler. 1994. Proliferating dendritic cell progenitors in human blood. J. Exp. Med. 180: 83–93.
Romani, N., D. Reider, M. Heuer, S. Ebner, E. Kämpgen, B. Eibl, D. Niederwieser, and G. Schuler. 1996. Generation of mature dendritic cells from human blood: An improved method with special regard to clinical applicability. J. lmmunol. Methods 196: 137–151
Bender, A., M. Sapp, G. Schuler, R. M. Steinman, and N. Bhardwaj. 1996. Improved methods for the generation of dendritic cells from nonproliferating progenitors in human blood. J. Immunol. Methods 196: 121–135
Zhou, L.-J. and T. F. Tedder. 1995. Human blood dendritic cells selectively express CD83, a member of the immunoglobulin superfamily. J. Immunol. 154: 3821–3835.
Sallusto, F., M. Cella, C. Danieli, and A. Lanzavecchia. 1995. Dendritic cells use macropinocytosis and the mannose receptor to concentrate macromolecules in the major histocompatibility complex class II compartment: Downregulation by cytokines and bacterial products. J. Exp. Med. 182: 389–400.
Kämpgen, E., N. Koch, F. Koch, P. Stöger, C. Heufler, G. Schuler, and N. Romani. 1991. Class II major histocompatibility complex molecules of murine dendritic cells: Synthesis, sialylation of invariant chain, and antigen processing capacity are down-regulated upon culture. Proc. Natl. Acad. Sci. USA 88: 3014–3018.
Pure, E., K. Inaba, M. T. Crowley, L. Tardelli, M. D. Witmer-Pack, G. Ruberti, G. Fathman, and R. M. Steinman. 1990. Antigen processing by epidermal Langerhans cells correlates with the level of biosynthesis of major histocompatibility complex class II molecules and expression of invariant chain. J. Exp. Med. 172: 1459–1469.
Becker, D., A. B. Reske-Kunz, J. Knop, and K. Reske. 1991. Biochemical properties of MHC class II molecules endogenously synthesized and expressed by mouse Langerhans cells. Eur. J. Immunol. 21: 12–13
Stössel, H., F. Koch, E. Kämpgen, P. Stöger, A. Lenz, C. Heufler, N. Romani, and G. Schuler. 1990. Disappearance of certain acidic organelles (endosomes and Langerhans cell granules) accompanies loss of antigen processing capacity upon culture of epidermal Langerhans cells. J. Exp. Med. 172: 1471–1482.
Kleijmeer, M. J., V. M. J. Oorschot, and H. J. Geuze. 1994. Human resident Langerhans cells display h lysosomal compartment enriched in MHC class II. J. Invest. Dermatol. 103: 516–523.
Radmayr, C., G. Bock, A. Hobisch, H. Klocker, G. Bartsch, and M. Thurnher. 1995. Dendritic antigen-presenting cells from the peripheral blood of renal-cell-carcinoma patients. Int. J. Cancer 63: 627–632.
Pope, M., M. G. H. Betjes, H. Hirmand, L. Hoffman, and R. M. Steinman. 1995. Both dendritic cells and memory T lymphocytes emigrate from organ cultures of human skin and form distinctive dendritic-T-cell conjugates. J. Invest. Dermatol. 104: 11–17.
Heufler, C., F. Koch, U. Stanzl, G. Topar, M. Wysocka, G. Trinchieri, A. Enk, R. M. Steinman, N. Romani, and G. Schuler. 1996. Interleukin-12 is produced by dendritic cells and mediates T helper I development as well as interferon-gamma production by T helper 1 cells. Eur.1. Immunol. 26: 659–668.
Macatonia, S. E., N. A. Hosken, M. Litton, P. Vieira, C.-S. Hsieh, J. A. Culpepper, M. Wysocka, G. Trinchieri, K. M. Murphy, and A. O’Garra. 1995. Dendritic cells produce IL-12 and direct the development of Th I cells from naive CD4’ T cells. J. Immunol. 154: 5071–5079.
Koch, F., U. Stanzl, P. Jennewein, K. Janke, C. Heufler, E. Kämpgen, N. Romani, and G. Schuler. 1996. High level IL-12 production by murine dendritic cells: Upregulation via MHC class 11 and CD40 molecules and downregulation by IL-4 and IL-10. J. Exp. Med. 184: 741–746.
Cella, M., D. Scheidegger, K. Palmer-Lehmann, P. Lane, A. Lanzavecchia, and G. Alber. 1996. Ligation of CD40 on dendritic cells triggers production of high levels of interleukin-12 and enhances T cell stimulatory capacity: T-T help via APC activation. J. Exp. Med. 184: 747–752.
Shu, U., M. Kiniwa, C. Y. Wu, C. Maliszewski, N. Vezzio, J. Hakimi, M. Gately, and G. Delespesse. 1995. Activated T cells induce interleukin-12 production by monocytes via CD40–CD40ligand interaction. Eur J. Immunol. 25: 1125–1128.
Dummer, W., B. C. Bastian, N. Ernst. C. Schänzle, A. Schwaaf, and E. B. Bröcker. 1996. Interleukin-10 production in malignant melanoma: Preferential detection of IL-10-secreting tumor cells in metastatic lesions. Int. J. Cancer 66: 607–610.
Buelens, C., F. Willems, A. Delvaux, G. Piérard, J. P. Delville, T. Velu, and M. Goldman. 1995. Interleukin10 differentially regulates B7–1 (CD80) and B7–2 (CD86) expression on human peripheral blood dendritic cells. Eur J. Immunol. 25: 2668–2672.
Péguet-Navarro, J., C. Mouton, C. Caux. C. Dalbiez-Gauthier, J. Banchereau, and D. Schmitt. 1994. Interleukin-10 inhibits the primary allogeneic T cell response to human epidermal Langerhans cells. Eur J. In, munol. 24: 884–891.
Austyn, J. M. 1996. New insights into the mobilization and phagocytic activity of dendritic cells. J. Exp. Med. 183: 1287–1292.
Lukas, M., H. Stössel, L. Hefel, S. Imamura, P. Fritsch, N. T. Sepp, G. Schuler, and N. Romani. 1996. Human cutaneous dendritic cells migrate through dermal lymphatic vessel culture model. J. Invest. Dermatol. 106: 1293–1299.
Hsu, F. J., C. Benike, F. Fagnoni, T. M. Liles, D. Czerwinski, B. Taidi, E. G. Engleman, and R. Levy. 1996. Vaccination of patients with B-cell lymphoma using autologous antigen-pulsed dendritic cells. Nature Med. 2: 52–58.
Zhou, L. J. and T. F. Tedder. 1996. CD14` blood monocytes can differentiate into functionally mature CD83 dendritic cells. Proc. Natl. Acad. Sci. USA 93: 2588–2592.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Springer Science+Business Media New York
About this chapter
Cite this chapter
Schuler, G., Romani, N. (1997). Generation of Mature Dendritic Cells from Human Blood. In: Ricciardi-Castagnoli, P. (eds) Dendritic Cells in Fundamental and Clinical Immunology. Advances in Experimental Medicine and Biology, vol 417. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9966-8_2
Download citation
DOI: https://doi.org/10.1007/978-1-4757-9966-8_2
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-9968-2
Online ISBN: 978-1-4757-9966-8
eBook Packages: Springer Book Archive