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The Influence of Collagen, Fibronectin, and Laminin on the Maturation of Dendritic Cell Progenitors Propagated from Normal or Flt3-Ligand-Treated Mouse Liver

  • Maureen L. Drakes
  • Lina Lu
  • Hilary J. Mckenna
  • Angus W. Thomson
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 417)

Abstract

Dendritic cell (DC) progenitors can be propagated from normal mouse liver non-parenchymal cell suspensions in the presence of granulocyte/macrophage colony stimulating factor (GM-CSF). These cells strongly express CD45, CD11b, heat stable antigen and CD44 cell surface markers. They exhibit moderate to low levels of expression of mouse DC-restricted markers (DEC-205, 33D1 and N418 [CD11c]) as well as low levels of MHC class II and B7–2 molecules, features characteristic of “immature” mouse DC.1,2,3 We have shown previously that these cells can be induced to express a mature DC phenotype and function (allostimulatory activity) following exposure to type-I collagen1, a molecule that is spatially associated with DC in the portal triads of normal liver.4–7 Here we report that these liver DC progenitors can also be induced to maturation in the presence of other extracellular matrix (ECM) proteins, namely fibronectin and laminin. Fibronectin is present along hepatic sinusoids of the liver, while laminin is localized on basement membranes along bile duct systems and blood vessels.4

Keywords

Flt3 Ligand CD44 Cell Surface Marker Bile Duct System Mouse Dendritic Cell Uncoated Plate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    L Lu, J Woo, AS Rao, Y Li, SC Watkins, S Qian, TE Starzl, AJ Demetris, AW Thomson. Propagatio of dendritic cell progenitors from normal mouse liver using granulocyte/macrophage colony-stimulating factor and their maturational development in the presence of type-1 collagen. J Exp Med 1994; 179: 1823.PubMedCrossRefGoogle Scholar
  2. 2.
    L Lu, D McCaslin, TE Starzl, AW Thomson. Mouse bone marrow-derived dendritic cell progenitors (NLDC 145+, MHC class II+, B7-ldim B7–2) induce alloantigen-specific hyporesponsiveness in murine T lymphocytes. Transplantation 1995; 60: 1539.PubMedCrossRefGoogle Scholar
  3. 3.
    AW Thomson, L Lu, S Qian, F Fu, JJ Fung, TE Starzl. Immature dendritic cells induce hyporesponsiveness to alloantigens in vitro and prolong mouse cardiac allograft survival. In: JW Alexander, and RA Good (eds) Transplantation Tolerance Induction RG Landes Co, Austin 1996; p. 141.Google Scholar
  4. 4.
    T Takahashi, M Isemura, T Nakamura, S Matsui, Y Oyanagi, H Asakura. Immunolocalization of a fibronectin-binding proteoglycan (PG-P1) immunologically related to HSPG2/perlecan in normal and fibrotic human liver. J Hepatology 1994; 21: 500.CrossRefGoogle Scholar
  5. 5.
    B Steiniger, J Klempnauer, K Wonigeit. Phenotype and histological distribution of interstitial dendritic cells in the pancreas, liver, heart and kidney. Transplantation 1984; 38: 169.PubMedCrossRefGoogle Scholar
  6. 6.
    TCR Prickett, JL McKenzie, DNJ Hart. Characterization of interstitial dendritic cells in human liver. Transplantation 1988; 46: 754.PubMedCrossRefGoogle Scholar
  7. 7.
    A Martinez-Hernandez. The hepatic extracellular matrix. I. Electron immunohistochemical studies in normal rat liver. Lab Invest 1984; 51: 57.PubMedGoogle Scholar
  8. 8.
    K Brasel, HJ McKenna, PJ Morrissey, K Charrier, AE Morris, CC Lee, DE Williams, SD Lyman. Hematological effects of FLT3 ligand in vivo in mice. Blood 1996 In pressGoogle Scholar
  9. 9.
    E Maraskovsky, K Brasel, M Teepe, E Roux, K Shortman, SD Lyman, HJ McKenna. In vivo administration of flt3 ligand results in the generation of large numbers of dendritic cells in the lymphoid tissue of mice. J Exp Med 1996 In press.Google Scholar
  10. 10.
    S Miyake, T Sakurai, K Okumura, H Yagita. Identification of collagen and laminin receptor integrins on murine T lymphocytes. Eur J Immunol 1994; 24: 2000.Google Scholar
  11. 11.
    TC Tsaio, W Xia, GM Rodberg, CE Pinto, RL Kradin. Interferon-gamma and tumor necrosis factor-alpha promote the binding of dendritic cells to fibronectin. Pathobiology 1994; 62: 120.CrossRefGoogle Scholar
  12. 12.
    MJ Arthur. Degradation of matrix proteins in liver fibrosis. Pathology, Research & Practice 1994; 190: 825.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Maureen L. Drakes
    • 1
    • 2
  • Lina Lu
    • 1
    • 2
  • Hilary J. Mckenna
    • 4
  • Angus W. Thomson
    • 1
    • 2
    • 3
  1. 1.Thomas E. Starzl Transplantation InstituteUniversity of PittsburghUSA
  2. 2.Department of SurgeryUniversity of PittsburghUSA
  3. 3.Department Molecular Genetics and BiochemistryUniversity of PittsburghUSA
  4. 4.Immunex Research Development CorporationSeattleUSA

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