Chapter

Dendritic Cells in Fundamental and Clinical Immunology

Volume 417 of the series Advances in Experimental Medicine and Biology pp 97-103

Migration of Dendritic Cells in 3D-Collagen Lattices

Visualisation of Dynamic Interactions with the Substratum and the Distribution of Surface Structures via a Novel Confocal Reflection Imaging Technique
  • Matthias GunzerAffiliated withInstitute of Immunology, University of Witten/Herdecke
  • , Eckhart KämpgenAffiliated withDepartment of Dermatology, University of Würzburg
  • , Eva-B. BröckerAffiliated withDepartment of Dermatology, University of Würzburg
  • , Kurt S. ZänkerAffiliated withInstitute of Immunology, University of Witten/Herdecke
  • , Peter FriedlAffiliated withInstitute of Immunology, University of Witten/HerdeckeDepartment of Dermatology, University of Würzburg

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Abstract

Migration is an inherent and prominent quality of dendritic cells (DC)1,2. In the past years numerous studies have analysed this aspect of DC biology. Quantitative aspects of DC migration have been tested in whole animal assays using different methods such as lymphcannulation3 or fluorescence labelling of migrating cells4,5. Skin-explant emigration assays were applied for in vitro studies6,7. Single DC have been investigated migrating on plastic surfaces8,9. We describe here a novel method for the analysis of individual cells migrating within a 3D-collagen substratum. The technique applies and further extends a system previously described by Fried10 for the analysis of lymphocyte migration. Using viable or fixed cells, the simultaneous visualisation and 3D-reconstruction of dynamic cell-interactions with the surrounding collagen-environment as well as the distribution of structures on the cell surface allows a more detailed analysis of the cell biology underlying the process of DC migration.