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Regulation of CD44 Isoform Expression and CD44-Mediated Signaling in Human Dendritic Cells

  • Hélène Haegel-Kronenberger
  • Henri de la Salle
  • Alain Bohbot
  • Jérôme Galon
  • Eric Tartour
  • Jean-Pierre Cazenave
  • Daniel Hanau
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 417)

Abstract

Dendritic cells (DCs) express CD44, a cell surface receptor for the extracellular matrix ligand hyaluronate, involved in cell-cell interactions and cell migration. Besides the “standard” form of CD44, a variety of splice variants contain an additional extracellular region encoded by 10 “variable” exons termed vl to v10. The standard form of CD44 as well as variants containing exon v6 (CD44v6) are known to play important roles in the immune system, yet largely unexplored in the DC lineage. In this study, we examined the regulation of CD44 isoforms in human DCs derived from monocytes cultivated in the presence of GM-CSF and IL-4. We found that v3, v6 and v9 variants are all up-regulated upon TNF-α stimulation of DCs. In addition, we show that stimulation of DCs using antiCD44 mAbs can induce DC agregation, up-regulation of accessory molecule expression and secretion of cytokines. A mAb directed against CD44v6 variants was shown to mediate some of these effects.

Keywords

Total CD44 Human Dendritic Cell CD44 Variant Variable Exon Variant Isoforms 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Hélène Haegel-Kronenberger
    • 1
  • Henri de la Salle
    • 1
  • Alain Bohbot
    • 2
  • Jérôme Galon
    • 3
  • Eric Tartour
    • 3
  • Jean-Pierre Cazenave
    • 4
  • Daniel Hanau
    • 1
  1. 1.INSERM CJF 94–03E.T.S.StrasbourgFrance
  2. 2.INSERM U.311E.T.S.StrasbourgFrance
  3. 3.Service d’Onco-HématologieHôpital de HautepierreStrasbourgFrance
  4. 4.INSERM U.255Institut CurieParisFrance

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