Advertisement

Biosynthetic Human Growth Hormone Identical to Authentic Material

  • Thorkild Christensen
  • Jørli W. Hansen
  • John Pedersen
  • Henrik Dalbøge
  • Søren Carlsen
  • Ejner B. Jensen
  • Karin D. Jørgensen
  • Bo Dinesen
  • Povl Nilsson
  • Hans H. Sørensen
  • Johannes Thomsen
  • Anne-Marie Kappelgaard
Part of the NATO ASI Series book series (NSSA, volume 125)

Abstract

The major component of human growth hormone (hGH) is a protein with 191 amino acid residues, and a molecular weight of approximately 22,000 D (22K-hGH). A minor component which constitutes about 5% of the more abundant form has a molecular weight of approximately 20,000 D. The minor form is derived from the major by deletion of 15 amino acid residues (32–46 of the 22K-isomer). Both molecules are single stranded, and two disulfide bridges stabilizes the structure. The N-terminus as well as the C-terminus is phenylalanine (Chawla et al., 1983).

Keywords

Human Growth Hormone Hydrophobic Interaction Chromatography Cyanogen Bromide High Performance Liquid Chromatogra Growth Hormone Deficient Child 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Aston, R., Cooper, L., Holder, A., Ivanyi, J., and Preece, M. (1985). Monoclonal antibodies to human growth hormone can distinguish between pituitary and genetically engineered forms. Molecular Immunology 22; 271.PubMedCrossRefGoogle Scholar
  2. Carlsen, S. and Jensen, E.B. (1986). Expression of amino extended human growth hormone in E.coli. Presented at Nordforsk Symposium 1986, Nyborg, Denmark, p. 56 (abstract).Google Scholar
  3. Chawla, R.K., Parks, J.S., and Rudman, D. (1983). Structural variants of human growth hormone: Biochemical, genetic and clinical aspects. Ann. Rev. Med. 34: 519.PubMedCrossRefGoogle Scholar
  4. Christensen, T., Hansen, J.J., Sørensen, H.H., and Thomsen, J. (1986). RP-HPLC of biosynthetic and hypophyseal human growth hormone in “High performance liquid chromatography in biotechnology”, ed. W.S. Hancock, John Wiley and Sons, Inc. (in press).Google Scholar
  5. Christiansen, J.S., Ørskov, H., Binder, C., and Kastrup, K.W. (1983). Imitation of normal plasma growth hormone profile by subcutaneous administration of human growth hormone to growth hormone deficient children. Acta Endocrinologica 102: 6.PubMedGoogle Scholar
  6. Dalbøge, H., Dahl, H-H.M., Pedersen, J., Hansen, J.W., and Christensen, T. (1986). A novel enzymatic method for production of authentic hGH from an E. coli produced hGH-precursor. Bio/Technology, accepted April 1986.Google Scholar
  7. Dinesen, B. and Andersen, H.D. (1984). Monitoring the production of biosynthetic human growth hormone by micro enzymelinked immunosorbent assay. Anal. Chim. Acta. 163: 119.CrossRefGoogle Scholar
  8. Gomez, F., Pirens, G., Schaus, C., Closset, J., and Hennen, G. (1984). A highly sensitive radioimmunoassay for human growth hormone using a monoclonal antibody. J. Immuno assay 5: 145.CrossRefGoogle Scholar
  9. Kastrup, K.W., Christiansen, J.S., Andersen, J.K., and Ørskov, H. (1983). Increased growth rate following transfer to daily sc administration from three weekly im injections of hGH in growth hormone deficient children. Acta Endocrinologica 104: 148.PubMedGoogle Scholar
  10. Urdea, M.S., Merryweather, J.P., Mullenbach, G.T., Coit, D., Heberlein, U., Valenzuela, P., and Barr, P.J. (1983). Chemical synthesis of a gene for human epidermal growth factor urogastrone and its expression in yeast. Proc. Natl. Acad. Sci. USA 80: 7461.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1986

Authors and Affiliations

  • Thorkild Christensen
    • 1
  • Jørli W. Hansen
    • 1
  • John Pedersen
    • 1
  • Henrik Dalbøge
    • 1
  • Søren Carlsen
    • 1
  • Ejner B. Jensen
    • 1
  • Karin D. Jørgensen
    • 1
  • Bo Dinesen
    • 1
  • Povl Nilsson
    • 1
  • Hans H. Sørensen
    • 1
  • Johannes Thomsen
    • 1
  • Anne-Marie Kappelgaard
    • 1
  1. 1.Nordisk Gentofte A/SGentofteDenmark

Personalised recommendations