Biosynthetic Human Growth Hormone Identical to Authentic Material
The major component of human growth hormone (hGH) is a protein with 191 amino acid residues, and a molecular weight of approximately 22,000 D (22K-hGH). A minor component which constitutes about 5% of the more abundant form has a molecular weight of approximately 20,000 D. The minor form is derived from the major by deletion of 15 amino acid residues (32–46 of the 22K-isomer). Both molecules are single stranded, and two disulfide bridges stabilizes the structure. The N-terminus as well as the C-terminus is phenylalanine (Chawla et al., 1983).
KeywordsHuman Growth Hormone Hydrophobic Interaction Chromatography Cyanogen Bromide High Performance Liquid Chromatogra Growth Hormone Deficient Child
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- Carlsen, S. and Jensen, E.B. (1986). Expression of amino extended human growth hormone in E.coli. Presented at Nordforsk Symposium 1986, Nyborg, Denmark, p. 56 (abstract).Google Scholar
- Christensen, T., Hansen, J.J., Sørensen, H.H., and Thomsen, J. (1986). RP-HPLC of biosynthetic and hypophyseal human growth hormone in “High performance liquid chromatography in biotechnology”, ed. W.S. Hancock, John Wiley and Sons, Inc. (in press).Google Scholar
- Dalbøge, H., Dahl, H-H.M., Pedersen, J., Hansen, J.W., and Christensen, T. (1986). A novel enzymatic method for production of authentic hGH from an E. coli produced hGH-precursor. Bio/Technology, accepted April 1986.Google Scholar