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Practical Determination of Control Coefficients in Metabolic Pathways

  • Enric I. Canela
  • Marta Cascante
  • Rafael Franco
Chapter
Part of the NATO ASI Series book series (NSSA, volume 190)

Abstract

It is obvious, not only for people working in metabolic control, that theory and practice can share common objectives but rarely share common roads, and it is often very difficult to put together in the same road theorists and experimentalists. In our opinion, metabolic control theories have also illustrated the difficulties in putting together these two disciplines: the hard nomenclature given by some control theories that is not easy for “test-tube” workers to follow; and the difficulties in directly measuring fluxes, intermediate concentrations or elasticity coefficients — terms introduced and popularized by metabolic control theory. In a given metabolic pathway, control of flux is exerted by the enzymes of the system. The direct calculation of flux sensitivities ∂lnJ/∂lnE i where J denotes a particular flux and E i the concentration of an enzyme, implies calculation of flux before and after a small addition of enzyme.This is often extremely difficult to achieve. With this regard indirect methods exist by which local properties (elasticity or special elasticity coefficients) are calculated first. Then control coefficients are calculated by using the equations corresponding to the theorems of metabolic control theory.

Keywords

Uric Acid Xanthine Oxidase Local Property Global Property Elasticity Coefficient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Brumen, M. and Heinrich, R. (1984) BioSystems 17, 155–169PubMedCrossRefGoogle Scholar
  2. Canela, E. I., Ginesta, I. and Franco, R. (1986) Arch. Biochem. Biophys. 254, 142–153CrossRefGoogle Scholar
  3. Cascante, M., Franco, R. and Canela, E. I. (1988) in Contrôle du metabolisme cellulaire (Mazat, J.-P. and Reder, C., eds.) pp. 53–63, BordeauxGoogle Scholar
  4. Cascante, M., Franco, R. and Canela, E. I. (1989a) Math. Biosci. 94,271–288Google Scholar
  5. Cascante, M., Franco, R. and Canela, E. I. (1989b) Math. Biosci. 94,289–309Google Scholar
  6. Fell, D. A. and Sauro, H. M. (1985) Eur. J. Biochem. 148, 155–161CrossRefGoogle Scholar
  7. Fell, D. A., Sauro, H. M. and Small, J. R. (1988) in Contrôle du metabolisme cellulaire (Mazat, J.-P. and Reder, C., eds.) pp. 15–26, BordeauxGoogle Scholar
  8. Fell, D. A. and Snell, K. (1988) Biochem. J. 256, 97–101.PubMedGoogle Scholar
  9. Flint, H. J., Porteous, D. J. and Kacser, H. (1980) Biochem. J 190, 1–15PubMedGoogle Scholar
  10. Flint, H. J., Tateson, R. W., Barthelmess, I. B., Porteous, D. J., Donachie, W. D. and Kacser, H. (1981) Biochem. J. 200, 155–161Google Scholar
  11. Franco, R. and Canela, E. I. (1984) Eur. J. Biochem. 144, 305–315PubMedCrossRefGoogle Scholar
  12. Groen, A. K., Van der Meer, R., Westerhoff, H. V., Wanders R. J., Akerboom, T. P. M. and Tager, J. M. (1982a) in Metabolic Compartmentation (Sies, H., ed.) pp. 9–37, Academic Press, New YorkGoogle Scholar
  13. Groen, A. K., Wanders R. J. A., Westerhoff, H. V., Van der Meer, R. and Tager, J. M. (1982b) J. Biol. Chem. 257, 2754–2757PubMedGoogle Scholar
  14. Groen, A. K., van Roermund, C. W. T., Vervoorn, R. C. and Tager, J. M. (1986) Biochem. J. 237, 379–389PubMedGoogle Scholar
  15. Heinrich, R. and Rapoport, S. M. (1983) Biochem. Soc. Trans. 11, 31–35PubMedGoogle Scholar
  16. Kacser, H. and Burns, J. A. (1979) Biochem. Soc. Trans. 7, 1149–1160PubMedGoogle Scholar
  17. Kacser, H. and Burns, J. A. (1981) Genetics 97, 639–666PubMedGoogle Scholar
  18. LaPorte, D. C., Walsh, K. and Koshland, D. E., Jr. (1984) J. Biol. Chem. 259, 14068–14075PubMedGoogle Scholar
  19. Mazat, J.-P., Jean-Bart, E., Rigoulet, M. and Guérin, B (1986) Biochim. Biophys. Acta 849, 7–15CrossRefGoogle Scholar
  20. Mazat, J.-P. and Reder, C. (eds.) (1988) Contrôle du metabolisme cellulaire, Bordeaux Middleton, R. J. and Kacser, H. (1983) Genetics 105, 633–650Google Scholar
  21. Rapoport, T. A., Heinrich, R., Jacobasch, G. and Rapoport, S. M. (1974) Eur JBiochem 42, 107–120CrossRefGoogle Scholar
  22. Rapoport, T. A. and Heinrich, R. (1975) BioSystems 7, 120–129PubMedCrossRefGoogle Scholar
  23. Rapoport, T. A., Heinrich, R. and Rapoport, S. M. (1976) Biochem. J.154,449–469Google Scholar
  24. Salter, M., Knowles, R. G. and Pogson, C. I. (1986) Biochem. J. 234, 635–647PubMedGoogle Scholar
  25. Savageau, M. A. (1976) Biochemical Systems Analysis: A Study of Function and Design in Molecular Biology, Addison-Wesley, Reading, MassachusettsGoogle Scholar
  26. Small, J. R. and Fell, D. A. (1989) J. Theor. Biol. 136, 181–197PubMedCrossRefGoogle Scholar
  27. Sorribas, A. and Baryons, R. (1986) Eur. J. Biochem. 158, 107–115PubMedCrossRefGoogle Scholar
  28. Stuart, F., Porteous, D. J., Flint, H. J. and Kacser, H (1986) J. Gen. Microbiol. 132, 1159–1166Google Scholar
  29. Tager, J. M., Groen, A. K., Wanders, R. J. A., Duszynski, J., Westerhoff, H. V. and Vervoom, R. C. (1983) Biochem. Soc. Trans. 11, 40–43PubMedGoogle Scholar
  30. Tones, N., Mateo, F., Meléndez-Hevia, E. and Kacser, H. (1986) Biochem. J. 234, 169–174Google Scholar
  31. Tones, N., Mateo, F. and Meléndez-Hevia, E. (1988a) FEBS Letters 233, 83–86CrossRefGoogle Scholar
  32. Tones, N., Mateo, F., Sicilia, J. and Meléndez-Hevia (1988b) Int. J. Biochem. 20, 161–165CrossRefGoogle Scholar
  33. Walsh, K. and Koshland, D. E., Jr. (1984) J. Biol. Chem. 259, 9649–9654Google Scholar
  34. Wanders, R. J. A., Meijer, A. J., van Roermund, C. M., Groen, A. K., Lof, V. and Tager, J. M. (1983) Biochem. Soc. Trans. 11, 89–90Google Scholar
  35. Wanders, R. J. A., van Roermund, C. W. T. and Meijer, A. J. (1984a) Eur. J. Biochem. 142, 247–254 Wanders, R. J. A., Groen, A. K., van Roermund, C. W. T. and Tager, J. M. (19846) Eur. J. Biochem. 142, 417–424Google Scholar
  36. Welch, G. R. (1977) Prog. Biophys. Molec. Biol. 32, 103–191CrossRefGoogle Scholar
  37. Werner, A. and Heinrich, R. (1985) Biomed. Biochim. Acta 44, 185–212Google Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Enric I. Canela
    • 1
  • Marta Cascante
    • 1
  • Rafael Franco
    • 1
  1. 1.Departament de Bioquímica i FisiologíaUniversitat de BarcelonaBarcelonaSpain

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