Respiratory Actions of Cholecystokinin and Its Interaction with Opioids at the Brainstem Level
CCK-8S may have an important role in the regulation of respiratory function. Immunohistochemical and autoradiographic studies have shown the presence of CCK-8-like immunoreactive terminals and CCK-8 receptors in several respiration related nuclei, such as the nucleus tractus solitarii (NTS) and the nucleus ambiguus (NA) (Zarbin et aí.,1983). Iontophoretic application of CCK-8S onto respiratory neurons in the NTS of cats, produced a decrease in their spontaneous activity and CCK-8S microinjected in the same nucleus depressed the phrenic activity in a reversible fashion (Denavit-Saubié et al., 1985). In contrast, the administration of the peptide into the brain ventricles of cats induced a potent stimulation of tidal volume (Gillis et al., 1983). The i.v. administration in decerebrated cats reduced the respiratory amplitude when the vagi were intact, but this effect was reversed to stimulation when they were sectioned (Hurlé et al., 1985). In order to assess the ability of CCK-8S to interact selectively with the brainstem respiratory structures, we have applied the peptide to the ventral medullary and to the dorso-rostral pontine surfaces of cats. The specificity of the effects has been tested by analyzing the antagonistic properties of proglumide, a CCK-8S antagonist.
KeywordsTidal Volume Respiratory Effect Minute Volume Nucleus Ambiguus Respiratory Neuron
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