Abstract
The phosphoinositides, namely phosphatidylinositol-4,5-bisphosphate (PIP2) and phosphatidylinositol (PI) play an influential role in intracellular signaling. Phospholipase C (PLC) hydrolyzes PIP2 and PI to produce the signaling molecules inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) which act to mobilize intracellular calcium and to stimulate protein phosphorylation, respectively.1 Quantifying PLC changes is thus one way to assess the phosphoinositide-dependent signal transduction process. We have previously reported that there are measurable amounts of PLC activity in the cat carotid body in the normoxic condition and that PLC activity is increased by hypoxia.2 Since activation of PLC may be part of the general mechanism by which the carotid body stimuli induce physiologic effects, we now extended that study by characterization of the effects on phosphoinositide metabolism of two further natural carotid body stimuli, respiratory and metabolic acidosis.
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References
E. Meldrum, P.J. Parker, and A. Carozzi, The PtdIns-PLC superfamily and signal transduction, Biochim. Biophys. Acta 1092: 49 (1991).
M. Pokorski and R. Strosznajder, PO2-dependence of phospholipase C in the cat carotid body, in: “Neurobiology and Cell Physiology,” P.G. Data, H. Acker, and S. Lahiri, ed., Plenum Press, London, (1992).
M. Pokorski and S. Lahiri, Aortic and carotid chemoreceptor responses to metabolic acidosis in the cat, Am. J. Physiol. 244:R652 (1983).
P.S. Rodrigues and J.E. Dowling, Dopamine induces neurite retraction in retinal horizontal cells via diacylglycerol and protein kinase C, Proc. Natl. Acad. Sci. 87:9693 (1990).
A. Gomez-Nino, B. Dinger, C. Gonzalez, and S.J. Fidone, Differential stimulus coupling to dopamine and norepinephrine stores in rabbit carotid body type I cells, Brain Res. 525:160 (1990).
R.H. Michell and C.J. Kirk, Studies of receptor-stimulated inositol lipid metabolism should focus upon measurements of inositol lipid breakdown, Biochem. J. 198:247 (1981).
N. Okamura, Y. Tajima, S. Onoe, and Y. Sugita, Purification of bicarbonate-sensitive sperm adenylylcyclase by 4-acetamido-4′-isothiocyanostilbene-2,2′-disulfonic acid-affinity chromatography, J. Biol. Chem. 266:17754 (1991).
W.J. Wang, G.F. Cheng, B.G. Dinger, and S.J. Fidone, Effects of hypoxia on cyclic nucleotide formation in rabbit carotid body in vitro, Neurosci. Lett. 105:164 (1989).
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© 1992 Springer Science+Business Media New York
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Pokorski, M., Strosznajder, R. (1992). Phosphoinositides and Signal Transduction in the Cat Carotid Body. In: Honda, Y., Miyamoto, Y., Konno, K., Widdicombe, J.G. (eds) Control of Breathing and Its Modeling Perspective. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9847-0_65
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DOI: https://doi.org/10.1007/978-1-4757-9847-0_65
Publisher Name: Springer, Boston, MA
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