A number of investigators have suggested a relationship between the evolutionary advancement of a vertebrate and the concentration and complexity of its serum proteins. This study measured total proteins and protein electrophoretic patterns in plasmas for 50 animal species representing the six major vertebrate classes. Paper electrophoresis employing sodium barbital buffer at pH 8.6 and ionic strength 0.05 was done on all the animals except the hedgehogs. Gel electrophoresis (Panagel) and immunoelectrophoresis (IEP) were also done on many of the animal plasmas and sera. Antisera to some of the animal plasmas were prepared in rabbits and used for the IEPs and for cross-immunoreactivity tests in agar gel.
KeywordsHuman Platelet Fibrin Degradation Product Fibrin Monomer Animal Plasma Mammalian Plasma
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