Interaction between Tyrosine Hydroxylase Expression and Olfactory Afferent Innervation in Embryonic Rat Olfactory Bulb in Vivo and in Vitro
A regulatory role for the olfactory bulb in the development and maintenance of the olfactory epithelium has been well documented. Removal of the olfactory bulb or sectioning of the olfactory nerves results in the death of mature receptor cells. Sensory cells are reconstituted from stem cells, found in the basal layer of the epithelium, which divide, differentiate and send out axons to reinnervate the olfactory bulb (Graziadei and Monti Graziadei, 1978). In vitro studies (Chuah and Farbman, 1983) have demonstrated that the presence of the olfactory bulb is necessary for the normal maturation of olfactory receptor neurons. Similarly, there is evidence for a role of the olfactory receptor innervation in the development of the olfactory bulb. Either deafferentation or odor deprivation in neonates results in abnormal development of the olfactory bulb, including reduced size and fewer neurons (for review, see Brunjes and Frazier, 1986). In addition, there is evidence for an inductive role of the olfactory afferent innervation. Supernumerary olfactory bulbs can be produced by transplantation of additional olfactory bulbs, and glomerular-like structures can be induced by regenerating afferent fibers which innervate the forebrain of bulbectomized mice (Graziadei et al., 1980; reviewed in Brunjes and Frazier, 1986).
KeywordsTyrosine Hydroxylase Olfactory Bulb Olfactory Receptor Olfactory Epithelium Main Olfactory Bulb
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