Cytosolic and Plasmamembranes Enzymatic Activities During Diethylnitrosamine-Induced Carcinogenesis in Rat Liver

  • Mario U. Dianzani
  • Rosa A. Canuto
  • Margherita Ferro
  • Anna M. Bassi
  • Luciana Paradisi
  • Maria E. Biocca
  • Giuliana Muzio

Abstract

It is well known that lipid peroxidation is strongly decreased in hepatomas with respect to normal liver and the extent of this decrease is proportional to that of dedifferentiation1,2.

Keywords

Cellulose Sucrose Glutathione Fluoride Oncol 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    M. U. Dianzani, R. A. Canuto, M. A. Rossi, G. Poli, R. Garcea, M. E. Biocca, G. Cecchini, F. Biasi, M. Ferro and A. M. Bassi, Further experiments on lipid peroxidation in transplanted and experimental hepatomas, Toxicol. Pathol. 12:189 (1984).PubMedCrossRefGoogle Scholar
  2. 2.
    T. J. Player, Lipid peroxidation in rat liver, hepatomas and regenerating liver, in: “Free Radicals, Lipid Peroxidation and Cancer”, D. C. H. McBrien and T. F. Slater, eds., Academic Press, London (1982).Google Scholar
  3. 3.
    E. Gravela, F. Feo, R. A. Canuto, R. Garcea and L. Gabriel, Functional and structural alteration of liver ergastoplasmic membranes during DL-ethionine hepatocarcinogenesis, Cancer Res. 35:3041 (1975).PubMedGoogle Scholar
  4. 4.
    M. U. Dianzani, G. Poli, R. A. Canuto, M. A. Rossi, M. E. Biocca, F. Biasi, G. Cecchini, G. Muzio, M. Ferro and H. Esterbauer, New data on kinetics of lipid peroxidation in experimental hepatomas and preneoplastic nodules, Toxicol. Pathol., 14:404 (1986).PubMedCrossRefGoogle Scholar
  5. 5.
    S. Hauptlorenz, H. Esterbauer, W. Moll, R. Pumpel, E. Schauenstein and B. Puschendorf, Effects of the lipid peroxidation product 4-hydroxynonenal and related aldehydes on proliferation and viability of cultured Ehrlich ascites tumor cells, Biochem. Pharmacol. 34:3803 (1985).PubMedCrossRefGoogle Scholar
  6. 6.
    M. U. Dianzani, Biochemical effects of saturated and unsaturated aldehyde, in: “Free Radicals, Lipid Peroxidation and Cancer”, D. C. H. McBrien and T. F. Slater, eds., Academic Press, London (1982).Google Scholar
  7. 7.
    L. Paradisi, C. Panagini, M. Parola, G. Barrera and M. U. Dianzani, Effects of 4-hydroxynonenal on adenylate cyclase and 5′-nucleotidase activities in rat liver plasmamembranes, Chem. Biol. Interact. 53:209 (1985).PubMedCrossRefGoogle Scholar
  8. 8.
    M. Curzio, H. Esterbauer, C. Di Mauro, G. Cecchini and M. U. Dianzani, Chemotactic activity of the lipid peroxidation product 4-hydroxynonenal and homologous hydroxyalkenals, Biol. Chem. Hopper-Seyler 367:321 (1986).CrossRefGoogle Scholar
  9. 9.
    G. Barrera, S. Martinotti, V. Fazio, V. Manzari, L. Paradisi, M. Parola, L. Frati and M. U. Dianzani, Effect of 4-hydroxynonenal on c-myc expression, Toxicol. Pathol. (1987) in press.Google Scholar
  10. 10.
    G. Barrera, V. M. Fazio, S. Martinotti, V. Manzari, M. G. Farace, M. U. Dianzani and L. Frati, Differenziazione ed inibizione di c-myc in cellule K562 attraverso prodotti delia lipoperossidazione cellulare, Atti V Congresso Nazionale ABCD, Sorrento (1986).Google Scholar
  11. 11.
    D. B. Solt, A. Medline and E. Farber, Rapid emergence of carcinogeninduced hyperplastic lesions in a new model for the sequential analysis of liver carcinogenesis, Amer. J. Pathol. 88:595 (1977).Google Scholar
  12. 12.
    M. W. Roomi, R. K. Ho, D. S. R. Sarma and E. Farber, A common biochemical pattern in preneoplastic hepatocyte nodules generated in four different models in the rat, Cancer Res. 45:564 (1985).PubMedGoogle Scholar
  13. 13.
    T. G. Flynn, Aldehyde reductases: monomeric NADPH-dependent oxidoreductases with multifunctional potential, Biochem. Pharmacol. 31:2705 (1982).PubMedCrossRefGoogle Scholar
  14. 14.
    H. Esterbauer, H. Zollner and J. Lang, Metabolism of the lipid peroxidation product 4-hydroxynonenal by isolated hepatocytes and by liver cytosolic fractions, Biochem. J. 228:363 (1985).PubMedGoogle Scholar
  15. 15.
    L. F. Chasseaud, The role of glutathione and glutathione-S-transferases in the metabolism of chemical carcinogens and other electophilic agents, Adv. Cancer Res. 29:175 (1979).PubMedCrossRefGoogle Scholar
  16. 16.
    S. O. C. Tottmar, H. Petterson and K. H. Kiessling, The subcellular distribution and properties of aldehyde dehydrogenase in rat liver, Biochem. J. 135:577 (1973).PubMedGoogle Scholar
  17. 17.
    R. Lindahl, Subcellular distribution and properties of aldehyde dehydrogenase from 2-acetylaminofluorene-induced rat hepatomas, Biochem. J. 183:55 (1979).PubMedGoogle Scholar
  18. 18.
    A. M. Rutenburg, H. Kim, J. W. Fishbein, H. C. Wassemberg and A. M. Seligman, Histochemical and ultrastructural demonstration of γ-glutamyltranspeptidase activity, J. Histochem. Cytochem. 17:517 (1969).PubMedCrossRefGoogle Scholar
  19. 19.
    A. H. Tashjian Jr., F. C. Bancroft, U. I. Richardson, M. B. Goldlust, F. A. Rommel and P. Ofner, Multiple differentiated functions in an unusual clonal strain of hepatoma cells, In Vitro 6:32 (1970).PubMedCrossRefGoogle Scholar
  20. 20.
    E. B. Thompson, G. M. Tomkins and J. F. Curran, Induction of tyrosine transaminase by steroid hormones in a newly established tissue culture cell line, Proc. Natl. Acad. Sci USA 56:296 (1966).PubMedCrossRefGoogle Scholar
  21. 21.
    G. Poli, E. Gravela, E. Albano and M. U. Dianzani, Studies on fatty liver with isolated hepatocytes. II. The action of carbon tetrachloride on lipid peroxidation, protein and triglyceride synthesis and secretion, Exp. Mol. Pathol. 30:116 (1979).PubMedCrossRefGoogle Scholar
  22. 22.
    R. A. Canuto, R. Garcea, M. E. Biocca, R. Pascale, L. Pirisi and F. Feo, The subcellular distribution and properties of aldehyde dehydrogenase of hepatoma AH-130, Eur. J. Cancer Clin. Oncol. 19:389 (1983).PubMedCrossRefGoogle Scholar
  23. 23.
    S. K. Srivastava, L. H. Ansari, G. A. Hair and B. Das, Aldose and aldehyde reductases in human tissues, Biochim. Biophys. Acta 800:220 (1984).PubMedCrossRefGoogle Scholar
  24. 24.
    P. Alin, U. H. Danielson and B. Mannervik, 4-hydroxyalk-2-enals are substrates for glutathione transferase, FEBS Lett. 179:267 (1985).PubMedCrossRefGoogle Scholar
  25. 25.
    A. G. Gornall, C. J. Bardawill and M. David, Determination of serum proteins by means of the biuret reaction, J. Biol. Chem. 177:751 (1949).PubMedGoogle Scholar
  26. 26.
    R. Lindahl and S. Evces, Changes in aldehyde dehydrogenase activity during diethylnitrosamine-initiated rat hepatocarcinogenesis, Carcinogenesis 8:785 (1987).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Mario U. Dianzani
    • 1
  • Rosa A. Canuto
    • 1
  • Margherita Ferro
    • 1
    • 2
  • Anna M. Bassi
    • 1
    • 2
  • Luciana Paradisi
    • 1
  • Maria E. Biocca
    • 1
  • Giuliana Muzio
    • 1
  1. 1.Dept. of Experimental Medicine and OncologyUniversity of TurinTurinItaly
  2. 2.Institute of General PathologyUniversity of GenoaGenoaItaly

Personalised recommendations