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Model Studies on the in Vitro Activation of c-Ha-ras Protooncogene by Dietary Components

  • Christine M. Ireland
  • Eric Hebert
  • Colin S. Cooper
  • David H. Phillips

Abstract

Evidence for the activation of ras protooncogenes by chemical carcinogens is provided both by the reproducible activation observed in chemically-induced animal tumors (reviewed in 1) and by our demonstration that in vitro modification of ras protooncogenes with ultimate carcinogens results in transformed foci on transfection into NIH3T3 cells2,3. In the present study, transforming oncogenes have been generated by the in vitro modification of plasmid containing human c-Ha-ras with the model ultimate carcinogens 3-N, N-acetoxyacetylamino-4,6-dimethyl-dipyrido[1,2-a:3′,2′-d]imidazole (N-AcO-AGlu-P-3) and 1′-acetoxysafrole (AcO-safrole). The mutations produced have been analyzed by selective amplification of the regions surrounding codons 12 and 61 and subsequent oligonucleotide hybridization.

Keywords

Chemical Carcinogen Single Base Pair B6C3F1 Mouse Base Pair Mismatch Ultimate Carcinogen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Christine M. Ireland
    • 1
  • Eric Hebert
    • 1
    • 2
  • Colin S. Cooper
    • 1
  • David H. Phillips
    • 1
  1. 1.Institute of Cancer ResearchChester Beatty LaboratoriesLondonUK
  2. 2.Centre de Biophysique MoleculaireCNRSOrleans, Cedex 2France

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