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Antibodies Specific for DNA Components Modified by Chemical Carcinogens and Their Binding Efficiency to DNA Modified in Vivo and in Vitro with the Corresponding Carcinogens

  • Erik Kriek
  • Frederik J. van Schooten
  • Michel J. X. Hillebrand
  • Maarten C. Welling

Abstract

It is now well established that almost all carcinogens form a variety of reaction products with DNA, involving covalent binding to the various nucleophilic sites on all four DNA bases as well as the phosphate groups of DNA. Thus, even qualitative determination of all DNA adducts, even from a single carcinogen, presents a formidable analytical task. Although the major reaction products of many carcinogens have been characterized (reviewed by Singer and Grunberger1), the structures of a large number of carcinogen-DNA adducts, particularly those of minor products, have not yet been identified. The quantitative determination is further complicated by the extremely low levels usually found in vivo, the removal of adducts from DNA by repair processes at different rates, and the formation of secondary lesions from chemically unstable adducts. Also, unstable adducts may be released spontaneously, leaving apurinic sites. Despite these shortcomings, measurement of DNA adducts is important, since these are thought to represent initiating events leading to mutations and/or malignant transformation. Measurement of DNA adducts in situ in the DNA of cells should give the most direct evidence of genotoxic exposure. The potential value of measuring carcinogen-DNA adducts as a dosimeter of human exposure has been discussed in a number of recent reviews2,3.

Keywords

Competitive ELISA Polycyclic Hydrocarbon Major Reaction Product Bovine Serum Albumin Conjugate Diol Epoxide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Erik Kriek
    • 1
  • Frederik J. van Schooten
    • 1
  • Michel J. X. Hillebrand
    • 1
  • Maarten C. Welling
    • 1
  1. 1.Division of Chemical CarcinogenesisThe Netherlands Cancer Institute (Antoni van Leeuwenhoek Huis)AmsterdamThe Netherlands

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