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In Situ Detection of Hepatic AF-DNA Adduct Formation During Continuous Feeding of 0.02% Acetylaminofluorene and in Enzyme-Altered Foci Induced by Foyr Different Protocols

  • Henrik S. Huitfeldt
  • Henry C. Pitot
  • John M. Hunt
  • Jeffrey Baron
  • Miriam C. Poirier

Abstract

Formation of carcinogen-DNA adducts is thought to represent the critical event in the initiation of tumorigenesis in many carcinogenesis models. DNA-adduct formation and repair has therefore been profoundly studied, both for human monitoring of carcinogen exposure and in experimental carcinogenesis. Most studies on DNA-adducts are based upon extraction of DNA from whole tissue, with little possibility to distinguish between different cell populations. The ability to differentiate between subpopulations within the tissue is presumably important, both to assess adduct processing within the tumor progenitor cells, and to monitor biological alterations of initiated cell populations.

Keywords

Adduct Formation Liver Lobule Continuous Feeding Bile Duct Proliferation Bile Duct Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Henrik S. Huitfeldt
    • 1
  • Henry C. Pitot
    • 2
  • John M. Hunt
    • 3
  • Jeffrey Baron
    • 4
  • Miriam C. Poirier
    • 5
  1. 1.Laboratory for Immunohistochemistry and Immunopathology Institute of PathologyThe National Hospital, University of OsloNorwayUSA
  2. 2.McArdle Laboratory for Cancer ResearchUniversity of WisconsinMadisonUSA
  3. 3.Department of Pathology and Laboratory MedicineUniversity of Texas Medical SchoolHoustonUSA
  4. 4.Department of Pharmacology, College of MedicineUniversity of IowaIowa CityUSA
  5. 5.Laboratory of Cellular Carcinogenesis and Tumor Promotion National Cancer InstituteNIHBethesdaUSA

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