In Situ Detection of Hepatic AF-DNA Adduct Formation During Continuous Feeding of 0.02% Acetylaminofluorene and in Enzyme-Altered Foci Induced by Foyr Different Protocols
Formation of carcinogen-DNA adducts is thought to represent the critical event in the initiation of tumorigenesis in many carcinogenesis models. DNA-adduct formation and repair has therefore been profoundly studied, both for human monitoring of carcinogen exposure and in experimental carcinogenesis. Most studies on DNA-adducts are based upon extraction of DNA from whole tissue, with little possibility to distinguish between different cell populations. The ability to differentiate between subpopulations within the tissue is presumably important, both to assess adduct processing within the tumor progenitor cells, and to monitor biological alterations of initiated cell populations.
KeywordsAdduct Fluor Hunt Biotin Phenobarbital
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- 2.M. C. Poirier, J. Nakayama, F. P. Perera, I. B. Weinstein and S. H. Yuspa, Identification of carcinogen-DNA adducts by immunoassays, in: “Application of Biological Markers to Carcinogen Testing”, H. A. Milman and S. Sell, eds., Plenum Press, New York (1983).Google Scholar
- 3.H. Holthofer, A. Miettinen, R. Paasivuo, V. P. Lehto, E. Linder, O. Alfthan and I. Virtanen, Cellular origin and differentiation of renal carcinomas. A fluorescence microscopic study with kidney-specific antibodies, anti-intermediate filament antibodies and lectins, Lab. Invest. 49:317 (1983).PubMedGoogle Scholar
- 7.H. A. Campbell, H. C. Pitot, V. R. Potter and B. A. Laishes, Application of quantitative stereology to the evaluation of enzyme-altered foci in rat liver, Cancer Res. 41:465 (1982).Google Scholar
- 9.H. P. Glauert, D. Beer, M. Sambasiva Rao, M. Schwarz, Y-D. Xu, T. L. Goldsworthy and H. C. Pitot, Induction of altered hepatic foci in rats by the administration of hypolipidemic peroxisome proliferators alone or following a single dose of diethylnitrosamine, Cancer Res. 46:4601 (1986).PubMedGoogle Scholar