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Characterization of a Transient intermediate in the Folding of Dihydrofolate Reductase

  • C. Robert Matthews
  • Edward P. Garvey
  • Jonathan Swank
Part of the Industry-University Cooperative Chemistry Program Symposia book series (IUCC)

Abstract

The central dogma of molecular biology describes the flow of genetic information from DNA to RNA (transcription) and from RNA to protein (translation). Although the information is stored and expressed in a linear format, the final product spontaneously folds to a unique three dimensional structure which is required for function. The past three decades have seen tremendous advances in our understanding of the processes of transcription and translation, however, rather little is known about the details of how the amino sequence of a protein directs the rapid and efficient folding to the native conformation.

Keywords

Tryptophan Residue Dihydrofolate Reductase Native Conformation Solvent Exposure Hydrophobic Cluster 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Fisher, G. and Schmid, F. X. (1990) Biochemistry 29, 2205–2212.CrossRefGoogle Scholar
  2. 2.
    Kim, P. S. and Baldwin, R. L. (1982) Annu. Rev. Biochem. 51, 459–489.PubMedCrossRefGoogle Scholar
  3. 3.
    Goldenberg, D. P. (1988) Annu. Rev. Biophys. Biophys. Chem. 17, 481–507.PubMedCrossRefGoogle Scholar
  4. 4.
    Touchette, N. A., Perry, K. M. and Matthews, C. R. (1986) Biochemistry 25, 5445–5452.PubMedCrossRefGoogle Scholar
  5. 5.
    Perry, K. M., Onuffer, J. J., Touchette, N. A., Herndon, C. S., Gittelman, M. S., Matthews, C. R., Chen, J.-T., Mayer, R. J., Taira, K., Benkovic, S. J., Howell, E. E. and Kraut, J. (1987) Biochemistry 26, 2674–2682.PubMedCrossRefGoogle Scholar
  6. 6.
    Garvey, E. P. and Matthews, C. R. (1989) Proteins 6, 259–266.PubMedCrossRefGoogle Scholar
  7. 7.
    Garvey, E. P. and Matthews, C. R. (1989) Biochemistry 28, 2083–2093.PubMedCrossRefGoogle Scholar
  8. 8.
    Bolin, J. T., Filman, D. J., Matthews, D. A., Hamlin, R. C. and Kraut, J. (1982) J. Biol. Chem. 257, 13650–13662.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • C. Robert Matthews
    • 1
  • Edward P. Garvey
    • 2
  • Jonathan Swank
    • 3
  1. 1.Department of ChemistryPennsylvania State UniversityUniversity ParkUSA
  2. 2.Division of Experimental TherapyBurroughs-Wellcome CorporationResearch Triangle ParkUSA
  3. 3.Medical College of PennsylvaniaPhiladelphiaUSA

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