Effect of Metal Ions and Adenylylation State on the Energetics of the E. Coli Glutamine Synthetase Reaction
Glutamine synthetase (GS) from E. coli requires two divalent metal ions per active site in order to catalyze the ATP-dependent formation of glutamine from glutamate and ammonia and plays a central role in ammonia metabolism in both prokaryotic and eukaryotic systems.
KeywordsGlutamine Synthetase Association Rate Internal Equilibrium Burst Rate Biosynthetic Reaction
- GS n=2
low adenylylation state enzyme
- GS n=12
high adenylylation state enzyme
N-(2-hydroxyethyl)piperazine-N′ -2-ethanesulfonic acid
ethylene diamine tetraacetic acid
piperazine-N, N′ -bis(2-ethanesulfonic acid)
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