Proteases of Isolated Pancreatic Acinar Cells after Caerulein Hyperstimulation
Acute pancreatitis has been considered for a long time as autodigestive disease of the pancreatic gland. However, the pathophysiology of this disease is still poorly understood. Therefore, some aspects of the pathogenesis may be investigated in experimental animal models. Supramaximal doses of the cholecystokinin analogue caerulein induces a mild edematous pancreatitis in rats . As main intracellular alterations, an impaired stimulus-secretion coupling, a formation of vacuolic structures, a diminished energy metabolism, and an activation of zymogens occur. A morphological feature of caerulein pancreatitis is the loss (or shedding) of the apical part of the plasma membrane. In the present study, this model was used to study the role of proteolytic enzymes to the pathological alterations of the pancreatic acinar cell. To this end, the activities of the potential cell surface peptidases dipeptidyl peptidase IV (DP IV) and aminopeptidase N (AP N) were measured in the course of caerulein pancreatitis and the following regeneration period. In a second set of experiments, the possible role of trypsin for initiating proteolytic actions within the acinar cell was investigated. For this purpose, we used rhodamine 110-based fluorogenic substrates to register proteolytic activities in intact cells.
KeywordsAcute Pancreatitis Serine Protease Acinar Cell Pancreatic Acinar Cell Pancreatic Acinus
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