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A Method to Investigate a Metabolic Process in a Single Neuron and Its Utilization in the Study of Fast Axonal Transport of Acetylcholine in a Cholinergic Neuron of Aplysia

  • Hiroyuki Koike
  • Yoshitomo Umitsu
  • Hiroko Matsumoto

Abstract

Intracellular recording techniques have been used successfully to analyze the electrical activities of specific single neurons among numerous heterogeneous populations of cells in the central nervous system. We have pursued development of a technique for studying the metabolic processes of single neurons by intracellular injection of radioactive substances via double-barreled Pyrex® capillary micropipettes (Koike et al., 1972; Koike and Tsuda, 1979, 1980; Koike and Matsumoto, 1985). This simple idea met several technical difficulties. (1) How could sufficient amounts of radioactive markers be applied into the soma of a single neuron? (2) How could we know whether all the materials injected were utilized for the metabolism in the neuron or not? (3) How could we eliminate inherent artifacts for this method such as leakage from the cell and reuptake of the leakage into nearby cells as well as intracellular diffusion of the injected substance. Now most of these difficulties have been overcome except for an inherent problem of time-consuming analyses resulting from single injections.

Keywords

Axonal Transport Cholinergic Neuron Radioactive Substance Diffusion Curve Intracellular Injection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Hiroyuki Koike
    • 1
  • Yoshitomo Umitsu
    • 1
  • Hiroko Matsumoto
    • 1
  1. 1.Department of NeurophysiologyTokyo Metropolitan Institute for Neurosciences, Fuchu CityTokyo 183Japan

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