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New Approach to the Study of Transport of Biomolecules by Microcarrier Cell Cultures Perfused in a Column Applying a High Resolution Paired-Tracer Technique

  • S. Perán
  • M. Muñoz
  • M. T. Saiz

Abstract

The functional and structural asymmetry of the plasma membrane makes the net uptake of any substrate the result of an interaction between two vectorial components of different magnitudes: one directed to the inside and another one directed to the outside of the cell (Stein, 1986). At the same time, the physiological activity of the cell determines membrane transport not as an isolated process with a finality in itself, but as one step of the metabolic pathways to which is coupled and from which is in many instances dependent. Hence, the mechanisms regulating influx and efflux must be directional. On the one hand, to meet the cellular requirements in response to internal stimuli and, on the other hand, to preserve the functional integrity of the organism responding to extracellular humoral and/or tissue (paracrine) stimuli (Shotwell et al., 1983).

Keywords

Human Fibroblast Amino Acid Transport Thermostated Column Microcarrier Culture Amino Acid Transport System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • S. Perán
    • 1
  • M. Muñoz
    • 1
  • M. T. Saiz
    • 1
  1. 1.Membrane Transport Group, Department of Biochemistry and Molecular Biology, Faculty of MedicineUniversity of MálagaMálagaSpain

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